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. 1991 Jul;57(7):2094–2096. doi: 10.1128/aem.57.7.2094-2096.1991

Hyphomonas spp., Shewanella spp., and Other Marine Bacteria Lack Heterogeneous (Ladderlike) Lipopolysaccharides

Darren D Sledjeski 1, Ronald M Weiner 1,*
PMCID: PMC183528  PMID: 16348529

Abstract

The lipopolysaccharides (LPS) of 19 marine bacteria were examined for size heterogeneities by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis in conjunction with an LPS-specific silver staining method. Fifteen marine bacteria had an R-type LPS instead of the ladderlike LPS array characteristic of most bacteria. In addition, three marine bacteria also had a single large LPS molecule. Without constraints (e.g., surface masking), R-type LPS, a more hydrophobic molecule, predominates in Shewanella and Hyphomonas species and in other marine bacteria.

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Selected References

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  1. Carlson R. W. Heterogeneity of Rhizobium lipopolysaccharides. J Bacteriol. 1984 Jun;158(3):1012–1017. doi: 10.1128/jb.158.3.1012-1017.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlson R. W., Kalembasa S., Turowski D., Pachori P., Noel K. D. Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development. J Bacteriol. 1987 Nov;169(11):4923–4928. doi: 10.1128/jb.169.11.4923-4928.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chart H., Shaw D. H., Ishiguro E. E., Trust T. J. Structural and immunochemical homogeneity of Aeromonas salmonicida lipopolysaccharide. J Bacteriol. 1984 Apr;158(1):16–22. doi: 10.1128/jb.158.1.16-22.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ciesielski C. A., Blaser M. J., Wang W. L. Serogroup specificity of Legionella pneumophila is related to lipopolysaccharide characteristics. Infect Immun. 1986 Feb;51(2):397–404. doi: 10.1128/iai.51.2.397-404.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldman R. C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem. 1980;107(1):145–153. doi: 10.1111/j.1432-1033.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
  6. Gustafsson B., Holme T. Immunological characterization of Vibrio cholerae O:1 lipopolysaccharide, O-side chain, and core with monoclonal antibodies. Infect Immun. 1985 Aug;49(2):275–280. doi: 10.1128/iai.49.2.275-280.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hitchcock P. J., Brown T. M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983 Apr;154(1):269–277. doi: 10.1128/jb.154.1.269-277.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jann B., Reske K., Jann K. Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur J Biochem. 1975 Dec 1;60(1):239–246. doi: 10.1111/j.1432-1033.1975.tb20996.x. [DOI] [PubMed] [Google Scholar]
  9. Meissner J., Pfennig N., Krauss J. H., Mayer H., Weckesser J. Lipopolysaccharides of Thiocystis violacea, Thiocapsa pfennigii, and Chromatium tepidum, species of the family Chromatiaceae. J Bacteriol. 1988 Jul;170(7):3217–3222. doi: 10.1128/jb.170.7.3217-3222.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Munford R. S., Hall C. L., Rick P. D. Size heterogeneity of Salmonella typhimurium lipopolysaccharides in outer membranes and culture supernatant membrane fragments. J Bacteriol. 1980 Nov;144(2):630–640. doi: 10.1128/jb.144.2.630-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nikaido H., Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol Rev. 1985 Mar;49(1):1–32. doi: 10.1128/mr.49.1.1-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Palva E. T., Mäkelä P. H. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Eur J Biochem. 1980;107(1):137–143. doi: 10.1111/j.1432-1033.1980.tb04634.x. [DOI] [PubMed] [Google Scholar]
  13. Parr T. R., Jr, Bryan L. E. Lipopolysaccharide banding patterns of Neisseria meningitidis and Neisseria gonorrhoeae. J Clin Microbiol. 1984 Apr;19(4):558–560. doi: 10.1128/jcm.19.4.558-560.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perez Perez G. I., Blaser M. J. Lipopolysaccharide characteristics of pathogenic campylobacters. Infect Immun. 1985 Feb;47(2):353–359. doi: 10.1128/iai.47.2.353-359.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Perez G. I., Hopkins J. A., Blaser M. J. Antigenic heterogeneity of lipopolysaccharides from Campylobacter jejuni and Campylobacter fetus. Infect Immun. 1985 May;48(2):528–533. doi: 10.1128/iai.48.2.528-533.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Preston M. A., Penner J. L. Structural and antigenic properties of lipopolysaccharides from serotype reference strains of Campylobacter jejuni. Infect Immun. 1987 Aug;55(8):1806–1812. doi: 10.1128/iai.55.8.1806-1812.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Russell R. R., Johnson K. G. SDS-polyacrylamide gel electrophoresis of lipopolysaccharides. Can J Microbiol. 1975 Dec;21(12):2013–2018. doi: 10.1139/m75-289. [DOI] [PubMed] [Google Scholar]
  18. Sindhu S. S., Brewin N. J., Kannenberg E. L. Immunochemical analysis of lipopolysaccharides from free-living and endosymbiotic forms of Rhizobium leguminosarum. J Bacteriol. 1990 Apr;172(4):1804–1813. doi: 10.1128/jb.172.4.1804-1813.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]
  20. de Maagd R. A., Rao A. S., Mulders I. H., Goosen-de Roo L., van Loosdrecht M. C., Wijffelman C. A., Lugtenberg B. J. Isolation and characterization of mutants of Rhizobium leguminosarum bv. viciae 248 with altered lipopolysaccharides: possible role of surface charge or hydrophobicity in bacterial release from the infection thread. J Bacteriol. 1989 Feb;171(2):1143–1150. doi: 10.1128/jb.171.2.1143-1150.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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