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. 1991 Aug;57(8):2324–2331. doi: 10.1128/aem.57.8.2324-2331.1991

Comparison of methods for discrimination between strains of Listeria monocytogenes from epidemiological surveys.

A O Baloga 1, S K Harlander 1
PMCID: PMC183571  PMID: 1662932

Abstract

Total cellular DNA from 28 strains of Listeria monocytogenes isolated from food implicated in food-borne illness and from patients with listeriosis was digested with the restriction endonucleases HindIII, HaeIII, and EcoRI. Following agarose gel electrophoresis, the fragments were subjected to Southern blot hybridization with a digoxigenin-labeled cDNA probe transcribed from Escherichia coli 16S and 23S rRNA. The patterns of bands from genomic (DNA fingerprints) and rDNA fingerprints (ribotypes) were used for classifying L. monocytogenes strains, and the resulting subtypes were compared with serotyping and multilocus enzyme electrophoresis classification schemes. A total of 15 distinct and identical groups were obtained when genomic DNA was digested with either HindIII or HaeIII. The most discriminating enzyme for ribotyping of strains was EcoRI, which divided the 28 strains of L. monocytogenes into 6 ribotype groups. DNA fingerprinting and ribotyping differentiated L. monocytogenes from other Listeria spp., including L. ivanovii, L. welshimeri, and L. innocua as well as the lactic acid bacteria Lactococcus lactis subsp. lactis and subsp. cremoris. L. monocytogenes strains isolated from four independent food-borne illness incidents were analyzed by all typing methods. Patient and product isolates were not distinguishable by serotyping, ribotyping, or multilocus enzyme electrophoresis. DNA fingerprinting was the only method capable of differentiating these strains, or conversely, of proving relatedness of patient-product pairs of isolates. This method was a relatively simple, sensitive, reproducible, and highly discriminating method for epidemiological tracking of L. monocytogenes implicated in food-borne illness.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allardet-Servent A., Bourg G., Ramuz M., Pages M., Bellis M., Roizes G. DNA polymorphism in strains of the genus Brucella. J Bacteriol. 1988 Oct;170(10):4603–4607. doi: 10.1128/jb.170.10.4603-4607.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bibb W. F., Gellin B. G., Weaver R., Schwartz B., Plikaytis B. D., Reeves M. W., Pinner R. W., Broome C. V. Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations. Appl Environ Microbiol. 1990 Jul;56(7):2133–2141. doi: 10.1128/aem.56.7.2133-2141.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bibb W. F., Schwartz B., Gellin B. G., Plikaytis B. D., Weaver R. E. Analysis of Listeria monocytogenes by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations. Int J Food Microbiol. 1989 Jun;8(3):233–239. doi: 10.1016/0168-1605(89)90018-4. [DOI] [PubMed] [Google Scholar]
  4. Bjorvatn B., Lund V., Kristiansen B. E., Korsnes L., Spanne O., Lindqvist B. Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis. J Clin Microbiol. 1984 Jun;19(6):763–765. doi: 10.1128/jcm.19.6.763-765.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Datta A. R., Wentz B. A., Hill W. E. Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization. Appl Environ Microbiol. 1987 Sep;53(9):2256–2259. doi: 10.1128/aem.53.9.2256-2259.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flamm R. K., Hinrichs D. J., Thomashow M. F. Cloning of a gene encoding a major secreted polypeptide of Listeria monocytogenes and its potential use as a species-specific probe. Appl Environ Microbiol. 1989 Sep;55(9):2251–2256. doi: 10.1128/aem.55.9.2251-2256.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  8. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  9. Gellin B. G., Broome C. V. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed] [Google Scholar]
  10. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  11. Kaper J. B., Bradford H. B., Roberts N. C., Falkow S. Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast. J Clin Microbiol. 1982 Jul;16(1):129–134. doi: 10.1128/jcm.16.1.129-134.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klinger J. D., Johnson A., Croan D., Flynn P., Whippie K., Kimball M., Lawrie J., Curiale M. Comparative studies of nucleic acid hybridization assay for Listeria in foods. J Assoc Off Anal Chem. 1988 May-Jun;71(3):669–673. [PubMed] [Google Scholar]
  13. Kuijper E. J., Oudbier J. H., Stuifbergen W. N., Jansz A., Zanen H. C. Application of whole-cell DNA restriction endonuclease profiles to the epidemiology of Clostridium difficile-induced diarrhea. J Clin Microbiol. 1987 Apr;25(4):751–753. doi: 10.1128/jcm.25.4.751-753.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Köhler S., Leimeister-Wächter M., Chakraborty T., Lottspeich F., Goebel W. The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes. Infect Immun. 1990 Jun;58(6):1943–1950. doi: 10.1128/iai.58.6.1943-1950.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Langenberg W., Rauws E. A., Widjojokusumo A., Tytgat G. N., Zanen H. C. Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J Clin Microbiol. 1986 Sep;24(3):414–417. doi: 10.1128/jcm.24.3.414-417.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linnan M. J., Mascola L., Lou X. D., Goulet V., May S., Salminen C., Hird D. W., Yonekura M. L., Hayes P., Weaver R. Epidemic listeriosis associated with Mexican-style cheese. N Engl J Med. 1988 Sep 29;319(13):823–828. doi: 10.1056/NEJM198809293191303. [DOI] [PubMed] [Google Scholar]
  17. McLauchlin J., Audurier A., Taylor A. G. The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies. J Med Microbiol. 1986 Dec;22(4):357–365. doi: 10.1099/00222615-22-4-357. [DOI] [PubMed] [Google Scholar]
  18. Nocera D., Bannerman E., Rocourt J., Jaton-Ogay K., Bille J. Characterization by DNA restriction endonuclease analysis of Listeria monocytogenes strains related to the Swiss epidemic of listeriosis. J Clin Microbiol. 1990 Oct;28(10):2259–2263. doi: 10.1128/jcm.28.10.2259-2263.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Notermans S., Chakraborty T., Leimeister-Wächter M., Dufrenne J., Heuvelman K. J., Maas H., Jansen W., Wernars K., Guinee P. Specific gene probe for detection of biotyped and serotyped Listeria strains. Appl Environ Microbiol. 1989 Apr;55(4):902–906. doi: 10.1128/aem.55.4.902-906.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Owen R. J., Beck A., Dayal P. A., Dawson C. Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons. J Clin Microbiol. 1988 Oct;26(10):2161–2166. doi: 10.1128/jcm.26.10.2161-2166.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Piffaretti J. C., Kressebuch H., Aeschbacher M., Bille J., Bannerman E., Musser J. M., Selander R. K., Rocourt J. Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. Proc Natl Acad Sci U S A. 1989 May;86(10):3818–3822. doi: 10.1073/pnas.86.10.3818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pérez-Díaz J. C., Vicente M. F., Baquero F. Plasmids in Listeria. Plasmid. 1982 Sep;8(2):112–118. doi: 10.1016/0147-619x(82)90049-x. [DOI] [PubMed] [Google Scholar]
  23. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  24. Schwartz B., Ciesielski C. A., Broome C. V., Gaventa S., Brown G. R., Gellin B. G., Hightower A. W., Mascola L. Association of sporadic listeriosis with consumption of uncooked hot dogs and undercooked chicken. Lancet. 1988 Oct 1;2(8614):779–782. doi: 10.1016/s0140-6736(88)92425-7. [DOI] [PubMed] [Google Scholar]
  25. Schwartz B., Hexter D., Broome C. V., Hightower A. W., Hirschhorn R. B., Porter J. D., Hayes P. S., Bibb W. F., Lorber B., Faris D. G. Investigation of an outbreak of listeriosis: new hypotheses for the etiology of epidemic Listeria monocytogenes infections. J Infect Dis. 1989 Apr;159(4):680–685. doi: 10.1093/infdis/159.4.680. [DOI] [PubMed] [Google Scholar]
  26. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  27. Szabo E. A., Desmarchelier P. M. A comparative study of clinical and food isolates of Listeria monocytogenes and related species. Epidemiol Infect. 1990 Oct;105(2):245–254. doi: 10.1017/s0950268800047853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Viering T. P., Fine D. P. Genetic analysis of Streptococcus pneumoniae serotypes with the use of DNA fingerprinting. J Infect Dis. 1989 Jul;160(1):76–82. doi: 10.1093/infdis/160.1.76. [DOI] [PubMed] [Google Scholar]
  30. Wachsmuth K. Molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks. Rev Infect Dis. 1986 Sep-Oct;8(5):682–692. doi: 10.1093/clinids/8.5.682. [DOI] [PubMed] [Google Scholar]
  31. Wesley I. V., Wesley R. D., Heisick J., Harrell F., Wagner D. Characterization of Listeria monocytogenes isolates by Southern blot hybridization. Vet Microbiol. 1990 Sep;24(3-4):341–353. doi: 10.1016/0378-1135(90)90182-u. [DOI] [PubMed] [Google Scholar]
  32. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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