Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Aug;57(8):2389–2391. doi: 10.1128/aem.57.8.2389-2391.1991

Pathogenicity of nonstressed, heat-stressed, and resuscitated Listeria monocytogenes 1A1 cells.

S A McCarthy 1
PMCID: PMC183580  PMID: 1768107

Abstract

The pathogenicity of nonstressed, heat-stressed, and resuscitated cells of Listeria monocytogenes 1A1 was assayed in immunocompromised mice. Cells were stressed by heating them at 56 degrees C for 20 min and were resuscitated by incubation in tryptic soy broth at 25 degrees C. A dose of 10(2) nonstressed and resuscitated cells per mouse was required for pathogenicity; a dose of 10(4) heat-stressed cells per mouse was considerably less pathogenic. Loss of hemolytic activity accompanied the decreased virulence.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beuchat L. R., Brackett R. E., Hao D. Y., Conner D. E. Growth and thermal inactivation of Listeria monocytogenes in cabbage and cabbage juice. Can J Microbiol. 1986 Oct;32(10):791–795. doi: 10.1139/m86-145. [DOI] [PubMed] [Google Scholar]
  2. Brackett R. E., Beuchat L. R. Pathogenicity of Listeria monocytogenes grown on crabmeat. Appl Environ Microbiol. 1990 May;56(5):1216–1220. doi: 10.1128/aem.56.5.1216-1220.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bunning V. K., Donnelly C. W., Peeler J. T., Briggs E. H., Bradshaw J. G., Crawford R. G., Beliveau C. M., Tierney J. T. Thermal inactivation of Listeria monocytogenes within bovine milk phagocytes. Appl Environ Microbiol. 1988 Feb;54(2):364–370. doi: 10.1128/aem.54.2.364-370.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cossart P., Vicente M. F., Mengaud J., Baquero F., Perez-Diaz J. C., Berche P. Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation. Infect Immun. 1989 Nov;57(11):3629–3636. doi: 10.1128/iai.57.11.3629-3636.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Czuprynski C. J., Brown J. F., Roll J. T. Growth at reduced temperatures increases the virulence of Listeria monocytogenes for intravenously but not intragastrically inoculated mice. Microb Pathog. 1989 Sep;7(3):213–223. doi: 10.1016/0882-4010(89)90057-0. [DOI] [PubMed] [Google Scholar]
  6. Dominguez Rodriguez L., Vazquez Boland J. A., Fernandez Garayzabal J. F., Echalecu Tranchant P., Gomez-Lucia E., Rodriguez Ferri E. F., Suarez Fernandez G. Microplate technique to determine hemolytic activity for routine typing of Listeria strains. J Clin Microbiol. 1986 Jul;24(1):99–103. doi: 10.1128/jcm.24.1.99-103.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Doyle M. P., Glass K. A., Beery J. T., Garcia G. A., Pollard D. J., Schultz R. D. Survival of Listeria monocytogenes in milk during high-temperature, short-time pasteurization. Appl Environ Microbiol. 1987 Jul;53(7):1433–1438. doi: 10.1128/aem.53.7.1433-1438.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farber J. M., Sanders G. W., Speirs J. I., D'Aoust J. Y., Emmons D. B., McKellar R. Thermal resistance of Listeria monocytogenes in inoculated and naturally contaminated raw milk. Int J Food Microbiol. 1988 Dec 31;7(4):277–286. doi: 10.1016/0168-1605(88)90054-2. [DOI] [PubMed] [Google Scholar]
  9. Farber J. M. Thermal resistance of Listeria monocytogenes in foods. Int J Food Microbiol. 1989 Jun;8(3):285–291. doi: 10.1016/0168-1605(89)90029-9. [DOI] [PubMed] [Google Scholar]
  10. Fernandez Garayzabal J. F., Dominguez Rodriguez L., Vazquez Boland J. A., Rodriguez Ferri E. F., Briones Dieste V., Blanco Cancelo J. L., Suarez Fernandez G. Survival of Listeria monocytogenes in raw milk treated in a pilot plant size pasteurizer. J Appl Bacteriol. 1987 Dec;63(6):533–537. doi: 10.1111/j.1365-2672.1987.tb02723.x. [DOI] [PubMed] [Google Scholar]
  11. Gellin B. G., Broome C. V. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed] [Google Scholar]
  12. Kathariou S., Rocourt J., Hof H., Goebel W. Levels of Listeria monocytogenes hemolysin are not directly proportional to virulence in experimental infections of mice. Infect Immun. 1988 Feb;56(2):534–536. doi: 10.1128/iai.56.2.534-536.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Portnoy D. A., Jacks P. S., Hinrichs D. J. Role of hemolysin for the intracellular growth of Listeria monocytogenes. J Exp Med. 1988 Apr 1;167(4):1459–1471. doi: 10.1084/jem.167.4.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roll J. T., Czuprynski C. J. Hemolysin is required for extraintestinal dissemination of Listeria monocytogenes in intragastrically inoculated mice. Infect Immun. 1990 Sep;58(9):3147–3150. doi: 10.1128/iai.58.9.3147-3150.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
  16. Sokolovic Z., Goebel W. Synthesis of listeriolysin in Listeria monocytogenes under heat shock conditions. Infect Immun. 1989 Jan;57(1):295–298. doi: 10.1128/iai.57.1.295-298.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stelma G. N., Jr, Reyes A. L., Peeler J. T., Francis D. W., Hunt J. M., Spaulding P. L., Johnson C. H., Lovett J. Pathogenicity test for Listeria monocytogenes using immunocompromised mice. J Clin Microbiol. 1987 Nov;25(11):2085–2089. doi: 10.1128/jcm.25.11.2085-2089.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES