Skip to main content
PMC home page
The BMJ logoLink to The BMJ
. 1989 Apr 8;298(6678):917–919. doi: 10.1136/bmj.298.6678.917

Survival of cultured allogeneic keratinocytes transplanted to deep dermal bed assessed with probe specific for Y chromosome.

A Brain 1, P Purkis 1, P Coates 1, M Hackett 1, H Navsaria 1, I Leigh 1
PMCID: PMC1836200  PMID: 2470447

Abstract

To determine the survival of cultured allogeneic keratinocytes transplanted to a deep dermal bed 24 tattoos that had been removed by deep shave excision in 19 patients were grafted with sheets of cultured allogeneic keratinocytes from donors of the opposite sex. Cells carrying the Y chromosome were identified in biopsy specimens taken from the graft site by in situ DNA hybridisation with a biotinylated Y probe (pHY 2.1) and visualised with a technique using immunoperoxidase. The cultured allograft sites were biopsied one, two, and three weeks after transplantation. No male cells were identified in any biopsy specimen from female patients who were given transplants of male cultured keratinocytes, and all biopsy specimens from male patients, who received female cultured keratinocytes, showed percentages of male cells within the normal range for male skin. The beneficial effects of cultivated allogeneic keratinocytes result from effects on wound healing other than forming a successful graft that "takes."

Full text

PDF
917

Images in this article

918p918-a
on p.918

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burns J., Chan V. T., Jonasson J. A., Fleming K. A., Taylor S., McGee J. O. Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells. J Clin Pathol. 1985 Oct;38(10):1085–1092. doi: 10.1136/jcp.38.10.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carter D. M., Lin A. N., Varghese M. C., Caldwell D., Pratt L. A., Eisinger M. Treatment of junctional epidermolysis bullosa with epidermal autografts. J Am Acad Dermatol. 1987 Aug;17(2 Pt 1):246–250. doi: 10.1016/s0190-9622(87)70199-6. [DOI] [PubMed] [Google Scholar]
  3. Coffey R. J., Jr, Derynck R., Wilcox J. N., Bringman T. S., Goustin A. S., Moses H. L., Pittelkow M. R. Production and auto-induction of transforming growth factor-alpha in human keratinocytes. 1987 Aug 27-Sep 2Nature. 328(6133):817–820. doi: 10.1038/328817a0. [DOI] [PubMed] [Google Scholar]
  4. Fabre J. W., Milton A. D., Spencer S., Settaf A., Houssin D. Regulation of alloantigen expression in different tissues. Transplant Proc. 1987 Feb;19(1 Pt 1):45–49. [PubMed] [Google Scholar]
  5. Gallico G. G., 3rd, O'Connor N. E., Compton C. C., Kehinde O., Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med. 1984 Aug 16;311(7):448–451. doi: 10.1056/NEJM198408163110706. [DOI] [PubMed] [Google Scholar]
  6. Gielen V., Faure M., Mauduit G., Thivolet J. Progressive replacement of human cultured epithelial allografts by recipient cells as evidenced by HLA class I antigens expression. Dermatologica. 1987;175(4):166–170. doi: 10.1159/000248820. [DOI] [PubMed] [Google Scholar]
  7. Grell R. F., Generoso E. E. A temporal study at the ultrastructural level of the developing pro-oocyte of Drosophila melanogaster. Chromosoma. 1982;87(1):49–75. doi: 10.1007/BF00333509. [DOI] [PubMed] [Google Scholar]
  8. Hall B. M. Cellular infiltrates in allografts. Transplant Proc. 1987 Feb;19(1 Pt 1):50–56. [PubMed] [Google Scholar]
  9. Hefton J. M., Amberson J. B., Biozes D. G., Weksler M. E. Loss of HLA-DR expression by human epidermal cells after growth in culture. J Invest Dermatol. 1984 Jul;83(1):48–50. doi: 10.1111/1523-1747.ep12261671. [DOI] [PubMed] [Google Scholar]
  10. Hefton J. M., Madden M. R., Finkelstein J. L., Shires G. T. Grafting of burn patients with allografts of cultured epidermal cells. Lancet. 1983 Aug 20;2(8347):428–430. doi: 10.1016/s0140-6736(83)90392-6. [DOI] [PubMed] [Google Scholar]
  11. Kedinger M., Haffen K., Grenier J., Eloy R. In vitro culture reduces immunogenicity of pancreatic endocrine islets. Nature. 1977 Dec 22;270(5639):736–738. doi: 10.1038/270736a0. [DOI] [PubMed] [Google Scholar]
  12. Kripke M. L. Immunological unresponsiveness induced by ultraviolet radiation. Immunol Rev. 1984 Aug;80:87–102. doi: 10.1111/j.1600-065x.1984.tb00496.x. [DOI] [PubMed] [Google Scholar]
  13. Lafferty K. J., Bootes A., Dart G., Talmage D. W. Effect of organ culture on the survival of thyroid allografts in mice. Transplantation. 1976 Aug;22(2):138–149. doi: 10.1097/00007890-197608000-00009. [DOI] [PubMed] [Google Scholar]
  14. Leigh I. M., Purkis P. E. Culture grafted leg ulcers. Clin Exp Dermatol. 1986 Nov;11(6):650–652. doi: 10.1111/j.1365-2230.1986.tb00526.x. [DOI] [PubMed] [Google Scholar]
  15. Leigh I. M., Purkis P. E., Navsaria H. A., Phillips T. J. Treatment of chronic venous ulcers with sheets of cultured allogenic keratinocytes. Br J Dermatol. 1987 Nov;117(5):591–597. doi: 10.1111/j.1365-2133.1987.tb07491.x. [DOI] [PubMed] [Google Scholar]
  16. Mackenzie I. C., Hill M. W. Connective tissue influences on patterns of epithelial architecture and keratinization in skin and oral mucosa of the adult mouse. Cell Tissue Res. 1984;235(3):551–559. doi: 10.1007/BF00226952. [DOI] [PubMed] [Google Scholar]
  17. Morhenn V. B., Benike C. J., Cox A. J., Charron D. J., Engleman E. G. Cultured human epidermal cells do not synthesize HLA-DR. J Invest Dermatol. 1982 Jan;78(1):32–37. doi: 10.1111/1523-1747.ep12497875. [DOI] [PubMed] [Google Scholar]
  18. Rheinwald J. G., Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975 Nov;6(3):331–343. doi: 10.1016/s0092-8674(75)80001-8. [DOI] [PubMed] [Google Scholar]
  19. Schultz G. S., White M., Mitchell R., Brown G., Lynch J., Twardzik D. R., Todaro G. J. Epithelial wound healing enhanced by transforming growth factor-alpha and vaccinia growth factor. Science. 1987 Jan 16;235(4786):350–352. doi: 10.1126/science.3492044. [DOI] [PubMed] [Google Scholar]

Articles from BMJ : British Medical Journal are provided here courtesy of BMJ Publishing Group

RESOURCES