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. 1982 Jul;22(1):162–164. doi: 10.1128/aac.22.1.162

Pyridinium-2-azo-p-dimethylaniline chromophore, a new chromogenic cephalosporin for rapid beta-lactamase testing.

J H Jorgensen, S A Crawford, G A Alexander
PMCID: PMC183694  PMID: 6982024

Abstract

A new chromogenic cephalosporin, pyridinium-2-azo-p-dimethylaniline chromophore, was evaluated for use in a rapid paper strip or tube test for the detection of beta-lactamases from a variety of microorganisms. A 1-min pyridinium-2-azo-p-dimethylaniline chromophore paper strip test was found to be a convenient and accurate method for the detection of beta-lactamase-producing strains of Haemophilus influenzae and Neisseria gonorrhoeae, although it could not be relied upon to detect beta-lactamases produced by staphylococci, enteric organisms, or Bacteroides fragilis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashford W. A., Golash R. G., Hemming V. G. Penicillinase-producing Neisseria gonorrhoeae. Lancet. 1976 Sep 25;2(7987):657–658. doi: 10.1016/s0140-6736(76)92467-3. [DOI] [PubMed] [Google Scholar]
  2. Bergström S., Norlander L., Norqvist A., Normark S. Contribution of a TEM-1-like beta-lactamase to penicillin resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1978 Apr;13(4):618–623. doi: 10.1128/aac.13.4.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catlin B. W. Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance. Antimicrob Agents Chemother. 1975 Mar;7(3):265–270. doi: 10.1128/aac.7.3.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Escamilla J. Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test. Antimicrob Agents Chemother. 1976 Jan;9(1):196–198. doi: 10.1128/aac.9.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jorgensen J. H., Lee J. C., Alexander G. A. Rapid penicillinase paper strip test for detection of beta-lactamase-producing Haemophilus influenzae and Neisseria gonorrhoeae. Antimicrob Agents Chemother. 1977 Jun;11(6):1087–1088. doi: 10.1128/aac.11.6.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Khan W., Ross S., Rodriguez W., Controni G., Saz A. K. Haemophilus influenzae type B resistant to ampicillin. A report of two cases. JAMA. 1974 Jul 15;229(3):298–301. [PubMed] [Google Scholar]
  7. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rosenblatt J. E., Neumann A. M. A rapid slide test for penicillinase. Am J Clin Pathol. 1978 Mar;69(3):351–354. doi: 10.1093/ajcp/69.1.351. [DOI] [PubMed] [Google Scholar]
  9. Thornsberry C., Anhalt J. P., Washington J. A., 2nd, McCarthy L. R., Schoenknecht F. D., Sherris J. C., Spencer H. J. Clinical laboratory evaluation of the Abbott MS-2 automated antimicrobial susceptibility testing system: report of a collaborative study. J Clin Microbiol. 1980 Sep;12(3):375–390. doi: 10.1128/jcm.12.3.375-390.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thornsberry C., Kirven L. A. Ampicillin resistance in Haemophilus influenzae as determined by a rapid test for beta-lactamase production. Antimicrob Agents Chemother. 1974 Nov;6(5):653–654. doi: 10.1128/aac.6.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]

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