Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1982 Oct;22(4):635–638. doi: 10.1128/aac.22.4.635

Antiviral activity and mechanism of action of 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (MDL-860).

H L Torney, J K Dulworth, D L Steward
PMCID: PMC183805  PMID: 7181474

Abstract

A nitrobenzene derivative, MDL-860, was found to inhibit plaque formation, cytopathic effect, or both in 11 of 12 picornaviruses at concentrations which did not affect cell growth. The compound did not directly inactivate the virus. MDL-860 inhibited actinomycin D-resistant [3H]uridine uptake in cells infected with coxsackievirus A21 or rhinovirus 1-A, whereas incorporation into uninfected cells was not inhibited. With three picornaviruses (echovirus type 12, poliovirus type 2, and rhinovirus type 1-A) made photosensitive with neutral red, MDL-860 did not appear to cause a significant reduction in their loss of photosensitivity (uncoating) during the first 3 h of infection. MDL-860 appears to inhibit some early event in virus replication, after uncoating, which is required for synthesis of a majority of viral RNA.

Full text

PDF
635

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Furman P. A., de Miranda P., St Clair M. H., Elion G. B. Metabolism of acyclovir in virus-infected and uninfected cells. Antimicrob Agents Chemother. 1981 Oct;20(4):518–524. doi: 10.1128/aac.20.4.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Mandel B. The relationship between penetration and uncoating of poliovirus in HeLa cells. Virology. 1967 Apr;31(4):702–712. doi: 10.1016/0042-6822(67)90198-5. [DOI] [PubMed] [Google Scholar]
  3. McSharry J. J., Caliguiri L. A., Eggers H. J. Inhibition of uncoating of poliovirus by arildone, a new antiviral drug. Virology. 1979 Sep;97(2):307–315. doi: 10.1016/0042-6822(79)90342-8. [DOI] [PubMed] [Google Scholar]
  4. Powers R. D., Gwaltney J. M., Jr, Hayden F. G. Activity of 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (MDL-860) against picornaviruses in vitro. Antimicrob Agents Chemother. 1982 Oct;22(4):639–642. doi: 10.1128/aac.22.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Wentworth B. B., French L. Plaque assay of Herpesvirus hominis on human embryonic fibroblasts. Proc Soc Exp Biol Med. 1969 Jun;131(2):588–592. doi: 10.3181/00379727-131-33932. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES