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. 1982 Oct;22(4):678–685. doi: 10.1128/aac.22.4.678

Tetracycline-resistant L-forms isolated from an antibiotic-susceptible strain of Listeria monocytogenes.

J Schmitt-Slomska, M C Marmouset, C Louis, R Bally, G Starka, S Madoff
PMCID: PMC183813  PMID: 6817706

Abstract

A tetracycline-susceptible strain of Listeria monocytogenes type 4b was converted to stable L-forms by penicillin. L-form variants resistant to tetracycline were then selected from a predominantly tetracycline-susceptible L-form population on plates containing penicillin and increasing concentrations of tetracycline. The origin of tetracycline-resistant L-forms from the parent Listeria strain was confirmed biochemically, by immunofluorescence, and by polyacrylamide gel electrophoresis. Scanning and transmission electron microscopy confirmed the typical L-form structure and the complete lack of cell wall in both L-form strains. The level of [3H]tetracycline uptake was lower in tetracycline-resistant than in susceptible cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball P. R., Shales S. W., Chopra I. Plasmid-mediated tetracycline resistance in Escherichia coli involves increased efflux of the antibiotic. Biochem Biophys Res Commun. 1980 Mar 13;93(1):74–81. doi: 10.1016/s0006-291x(80)80247-6. [DOI] [PubMed] [Google Scholar]
  2. Branton D., Bullivant S., Gilula N. B., Karnovsky M. J., Moor H., Mühlethaler K., Northcote D. H., Packer L., Satir B., Satir P. Freeze-etching nomenclature. Science. 1975 Oct 3;190(4209):54–56. doi: 10.1126/science.1166299. [DOI] [PubMed] [Google Scholar]
  3. Chopra I., Howe T. G. Bacterial resistance to the tetracyclines. Microbiol Rev. 1978 Dec;42(4):707–724. doi: 10.1128/mr.42.4.707-724.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eccles S., Docherty A., Chopra I., Shales S., Ball P. Tetracycline resistance genes from Bacillus plasmid pAB124 confer decreased accumulation of the antibiotic in Bacillus subtilis but not in Escherichia coli. J Bacteriol. 1981 Mar;145(3):1417–1420. doi: 10.1128/jb.145.3.1417-1420.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Espaze E. P., Roubeix Y. G., Le Berre J. Y., Courtieu A. I. In vitro susceptibility of Listeria monocytogenes to some antibiotics and their combinations. Zentralbl Bakteriol Orig A. 1978 Jan;240(1):76–85. [PubMed] [Google Scholar]
  6. Foster T. J. R factor tetracycline and chloramphenicol resistance in Escherichia coli K12 cmlB mutants. J Gen Microbiol. 1975 Oct;90(2):303–310. doi: 10.1099/00221287-90-2-303. [DOI] [PubMed] [Google Scholar]
  7. Franklin T. J., Higginson B. Active accumulation of tetracycline by Escherichia coli. Biochem J. 1970 Jan;116(2):287–297. doi: 10.1042/bj1160287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Franklin T. J. Resistance of Escherichia coli to tetracyclines. Changes in permeability to tetracyclines in Escherichia coli bearing transferable resistance factors. Biochem J. 1967 Oct;105(1):371–378. doi: 10.1042/bj1050371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horodniceanu T., Bouanchaud D. H., Bieth G., Chabbert Y. A. R plasmids in Streptococcus agalactiae (group B). Antimicrob Agents Chemother. 1976 Nov;10(5):795–801. doi: 10.1128/aac.10.5.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hubert E. G., Kalmanson G. M., Montgomerie J. Z., Guze L. B. Activity of methacycline, related tetracyclines, and other antibiotics against various L-forms and their parent bacteria in vitro. Antimicrob Agents Chemother. 1972 Oct;2(4):276–280. doi: 10.1128/aac.2.4.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kalvodová D. L-formy Listeria monocytogenes. Indukce in vitro a citlivost na antibiotika. Cesk Epidemiol Mikrobiol Imunol. 1974 Aug;23(4-5):276–281. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Levy S. B., McMurry L. Detection of an inducible membrane protein associated with R-factor-mediated tetracycline resistance. Biochem Biophys Res Commun. 1974 Feb 27;56(4):1060–1068. doi: 10.1016/s0006-291x(74)80296-2. [DOI] [PubMed] [Google Scholar]
  14. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  15. McMurry L., Levy S. B. Two transport systems for tetracycline in sensitive Escherichia coli: critical role for an initial rapid uptake system insensitive to energy inhibitors. Antimicrob Agents Chemother. 1978 Aug;14(2):201–209. doi: 10.1128/aac.14.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Michel G., Di Savino D., Starka J. Phospholipid composition and phenotypic correction of an envC division mutant of Escherichia coli. J Bacteriol. 1977 Jan;129(1):145–150. doi: 10.1128/jb.129.1.145-150.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moellering R. C., Jr, Medoff G., Leech I., Wennersten C., Kunz L. J. Antibiotic synergism against Listeria monocytogenes. Antimicrob Agents Chemother. 1972 Jan;1(1):30–34. doi: 10.1128/aac.1.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Montgomerie J. Z., Kalmanson G. M., Guze L. B. The effects of antibiotics on the protoplast and bacterial forms of Streptococcus faecalis. J Lab Clin Med. 1966 Oct;68(4):543–551. [PubMed] [Google Scholar]
  19. SHARP J. T. L Colonies from hemolytic streptocci: new technic in the study of L forms of bacteria. Proc Soc Exp Biol Med. 1954 Oct;87(1):94–97. doi: 10.3181/00379727-87-21297. [DOI] [PubMed] [Google Scholar]
  20. Schmitt-Slomska J., Caravano R., El-Solh N. Loss of plasmid-mediated resistance after conversion of a group B streptococcus strain to a stable cell wall deficient variant. Ann Microbiol (Paris) 1979 Jan;130 A(1):23–27. [PubMed] [Google Scholar]
  21. Shales S. W., Chopra I., Ball P. R. Evidence for more than one mechanism of plasmid-determined tetracycline resistance in Escherichia coli. J Gen Microbiol. 1980 Nov;121(1):221–229. doi: 10.1099/00221287-121-1-221. [DOI] [PubMed] [Google Scholar]
  22. Yamamoto A., Homma J. Y. L-form of Pseudomonas aeruginosa. 2. Antibiotic sensitivity of L-forms and their parent forms. Jpn J Exp Med. 1978 Aug;48(4):355–362. [PubMed] [Google Scholar]

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