Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Oct;57(10):2956–2962. doi: 10.1128/aem.57.10.2956-2962.1991

Distribution of plasmid- and chromosome-mediated iron uptake systems in Vibrio anguillarum strains of different origins.

R F Conchas 1, M L Lemos 1, J L Barja 1, A E Toranzo 1
PMCID: PMC183904  PMID: 1836126

Abstract

We investigated the incidence of plasmid-mediated and chromosome-mediated iron uptake systems in strains of Vibrio anguillarum that belong to serotypes O1 and O2 and were isolated from different fish species and in different geographic areas. All of the strains gave positive reactions in CAS agar medium and in the Arnow test, which indicated that catechol types of siderophores were produced. The majority of V. anguillarum serotype O1 strains harbored a 65-kb plasmid similar to plasmid pJM1 from strain 775, which encodes the siderophore anguibactin and its outer membrane receptor, protein OM2. All of the isolates harboring this plasmid promoted the growth of an anguibactin-deficient receptor-proficient mutant derived from strain 775, but none of these isolates promoted the growth of mutants lacking receptor OM2. Furthermore, under iron-limiting conditions all of these strains induced outer membrane proteins that were identical in size to protein OM2 of strain 775. In contrast, none of the serotype O2 strains contained a high-molecular-weight plasmid, but all of them induced the growth of mutants defective in the anguibactin-mediated system regardless of the presence or absence of receptor OM2. The serotype O2 strains, but not the plasmid-bearing serotype O1 strains, also induced the growth of Salmonella typhimurium enb-1 which utilizes only enterobactin as a siderophore.(ABSTRACT TRUNCATED AT 250 WORDS)

Full text

PDF
2956

Images in this article

2960Image
on p.2960
2960Image
on p.2960

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Actis L. A., Fish W., Crosa J. H., Kellerman K., Ellenberger S. R., Hauser F. M., Sanders-Loehr J. Characterization of anguibactin, a novel siderophore from Vibrio anguillarum 775(pJM1). J Bacteriol. 1986 Jul;167(1):57–65. doi: 10.1128/jb.167.1.57-65.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Actis L. A., Potter S. A., Crosa J. H. Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1. J Bacteriol. 1985 Feb;161(2):736–742. doi: 10.1128/jb.161.2.736-742.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crosa J. H. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature. 1980 Apr 10;284(5756):566–568. doi: 10.1038/284566a0. [DOI] [PubMed] [Google Scholar]
  4. Crosa J. H., Actis L. A., Mitoma Y., Perez-Casal J., Tolmasky M. E., Valvano M. A. Plasmid-mediated iron sequestering systems in pathogenic strains of Vibrio anguillarum and Escherichia coli. Basic Life Sci. 1985;30:759–774. doi: 10.1007/978-1-4613-2447-8_52. [DOI] [PubMed] [Google Scholar]
  5. Crosa J. H., Hodges L. L. Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775. Infect Immun. 1981 Jan;31(1):223–227. doi: 10.1128/iai.31.1.223-227.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crosa J. H., Hodges L. L., Schiewe M. H. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infect Immun. 1980 Mar;27(3):897–902. doi: 10.1128/iai.27.3.897-902.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Crosa J. H., Schiewe M. H., Falkow S. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infect Immun. 1977 Nov;18(2):509–513. doi: 10.1128/iai.18.2.509-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lemos M. L., Salinas P., Toranzo A. E., Barja J. L., Crosa J. H. Chromosome-mediated iron uptake system in pathogenic strains of Vibrio anguillarum. J Bacteriol. 1988 Apr;170(4):1920–1925. doi: 10.1128/jb.170.4.1920-1925.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Neilands J. B. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. doi: 10.1146/annurev.nu.01.070181.000331. [DOI] [PubMed] [Google Scholar]
  12. Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
  13. Olsen J. E., Larsen J. L. Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol. 1990 Oct;56(10):3130–3132. doi: 10.1128/aem.56.10.3130-3132.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pollack J. R., Ames B. N., Neilands J. B. Iron transport in Salmonella typhimurium: mutants blocked in the biosynthesis of enterobactin. J Bacteriol. 1970 Nov;104(2):635–639. doi: 10.1128/jb.104.2.635-639.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schwyn B., Neilands J. B. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987 Jan;160(1):47–56. doi: 10.1016/0003-2697(87)90612-9. [DOI] [PubMed] [Google Scholar]
  16. Sørensen U. B., Larsen J. L. Serotyping of Vibrio anguillarum. Appl Environ Microbiol. 1986 Mar;51(3):593–597. doi: 10.1128/aem.51.3.593-597.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tolmasky M. E., Actis L. A., Toranzo A. E., Barja J. L., Crosa J. H. Plasmids mediating iron uptake in Vibrio anguillarum strains isolated from turbot in Spain. J Gen Microbiol. 1985 Aug;131(8):1989–1997. doi: 10.1099/00221287-131-8-1989. [DOI] [PubMed] [Google Scholar]
  18. Tolmasky M. E., Salinas P. C., Actis L. A., Crosa J. H. Increased production of the siderophore anguibactin mediated by pJM1-like plasmids in Vibrio anguillarum. Infect Immun. 1988 Jun;56(6):1608–1614. doi: 10.1128/iai.56.6.1608-1614.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Toranzo A. E., Barja J. L., Potter S. A., Colwell R. R., Hetrick F. M., Crosa J. H. Molecular factors associated with virulence of marine vibrios isolated from striped bass in Chesapeake Bay. Infect Immun. 1983 Mar;39(3):1220–1227. doi: 10.1128/iai.39.3.1220-1227.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walter M. A., Potter S. A., Crosa J. H. Iron uptake system medicated by Vibrio anguillarum plasmid pJM1. J Bacteriol. 1983 Nov;156(2):880–887. doi: 10.1128/jb.156.2.880-887.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wiik R., Hoff K. A., Andersen K., Daae F. L. Relationships between plasmids and phenotypes of presumptive strains of Vibrio anguillarum isolated from different fish species. Appl Environ Microbiol. 1989 Apr;55(4):826–831. doi: 10.1128/aem.55.4.826-831.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wolf M. K., Crosa J. H. Evidence for the role of a siderophore in promoting Vibrio anguillarum infections. J Gen Microbiol. 1986 Oct;132(10):2949–2952. doi: 10.1099/00221287-132-10-2949. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES