Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Oct;57(10):3052–3055. doi: 10.1128/aem.57.10.3052-3055.1991

Use of Immunogold Labelling with Scanning Electron Microscopy To Identify Phytopathogenic Bacteria on Leaf Surfaces †

C L Davis 1, R H Brlansky 1,*
PMCID: PMC183922  PMID: 16348573

Abstract

Scanning electron microscopy coupled with high-resolution back-scattered electron imaging was used to detect gold-labelled specific immunoglobulins attached to epiphytic bacteria. Strains of Xanthomonas citri and X. campestris pv. citrumelo were specifically identified on grapefruit leaf surfaces when labelled with homologous gold-labelled immunoglobulins.

Full text

PDF
3052

Images in this article

3053Image
on p.3053

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews J. H., Kenerley C. M. The effects of a pesticide program on non-target epiphytic microbial populations of apple leaves. Can J Microbiol. 1978 Sep;24(9):1058–1072. doi: 10.1139/m78-175. [DOI] [PubMed] [Google Scholar]
  2. Horisberger M., Rosset J., Bauer H. Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope. Experientia. 1975 Oct 15;31(10):1147–1149. doi: 10.1007/BF02326761. [DOI] [PubMed] [Google Scholar]
  3. Horisberger M., Rosset J. Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem. 1977 Apr;25(4):295–305. doi: 10.1177/25.4.323352. [DOI] [PubMed] [Google Scholar]
  4. Leben C. Colonization of soybean buds by bacteria: observations with the scanning electron microscope. Can J Microbiol. 1969 Mar;15(3):319–320. doi: 10.1139/m69-059. [DOI] [PubMed] [Google Scholar]
  5. Miller T. J., Stone H. O. The rapid isolation of ribonuclease-free immunoglobulin G by protein A-sepharose affinity chromatography. J Immunol Methods. 1978;24(1-2):111–125. doi: 10.1016/0022-1759(78)90092-3. [DOI] [PubMed] [Google Scholar]
  6. Moon N. J., Henk W. G. Progression of epiphytic microflora in wheat and alfalfa silages as observed by scanning electron microscopy. Appl Environ Microbiol. 1980 Dec;40(6):1122–1129. doi: 10.1128/aem.40.6.1122-1129.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Trejdosiewicz L. K., Smolira M. A., Hodges G. M., Goodman S. L., Livingston D. C. Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence.. J Microsc. 1981 Aug;123(Pt 2):227–236. doi: 10.1111/j.1365-2818.1981.tb01297.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES