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. 1991 Nov;57(11):3385–3387. doi: 10.1128/aem.57.11.3385-3387.1991

Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus

Nami Touhara 1, Hayao Taguchi 1, Yoshinori Koyama 1, Takahisa Ohta 1, Hiroshi Matsuzawa 1,*
PMCID: PMC183977  PMID: 16348594

Abstract

Aqualysin I is synthesized as a large precursor, processed, and secreted into the culture medium by Thermus aquaticus YT-1. An expression plasmid for the aqualysin I gene in T. thermophilus HB27 was constructed. T. thermophilus cells harboring the recombinant plasmid produced correctly processed aqualysin I, and the mature enzyme was secreted into the culture medium.

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Selected References

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