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. 1991 Dec;57(12):3656–3659. doi: 10.1128/aem.57.12.3656-3659.1991

Separation of Methanotrophic Bacteria by Using Percoll and Its Application to Isolation of Mixed and Pure Cultures †

K P Putzer 1,‡, L A Buchholz 1, M E Lidstrom 1, C C Remsen 1,*
PMCID: PMC184030  PMID: 16348612

Abstract

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

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Selected References

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