Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1991 Dec;57(12):3677–3678. doi: 10.1128/aem.57.12.3677-3678.1991

Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.

L Benbadis 1, J R Garel 1, D L Hartley 1
PMCID: PMC184036  PMID: 1785940

Abstract

SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.

Full text

PDF
3677

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arber W. Host-controlled modification of bacteriophage. Annu Rev Microbiol. 1965;19:365–378. doi: 10.1146/annurev.mi.19.100165.002053. [DOI] [PubMed] [Google Scholar]
  2. Benbadis L., Faelen M., Slos P., Fazel A., Mercenier A. Characterization and comparison of virulent bacteriophages of Streptococcus thermophilus isolated from yogurt. Biochimie. 1990 Dec;72(12):855–862. doi: 10.1016/0300-9084(90)90002-x. [DOI] [PubMed] [Google Scholar]
  3. Boussemaer J. P., Schrauwen P. P., Sourrouille J. L., Guy P. Multiple modification/restriction systems in lactic streptococci and their significance in defining a phage-typing system. J Dairy Res. 1980 Oct;47(3):401–409. doi: 10.1017/s0022029900021294. [DOI] [PubMed] [Google Scholar]
  4. Brown N. L., Smith M. Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I). Proc Natl Acad Sci U S A. 1977 Aug;74(8):3213–3216. doi: 10.1073/pnas.74.8.3213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellis K. J., Morrison J. F. Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol. 1982;87:405–426. doi: 10.1016/s0076-6879(82)87025-0. [DOI] [PubMed] [Google Scholar]
  6. Fitzgerlad G. F., Daly C., Brown L. R., Gingeras T. R. ScrFI: a new sequence-specific endonuclease from Streptococcus cremoris. Nucleic Acids Res. 1982 Dec 20;10(24):8171–8179. doi: 10.1093/nar/10.24.8171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hill C., Miller L. A., Klaenhammer T. R. In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage. J Bacteriol. 1991 Jul;173(14):4363–4370. doi: 10.1128/jb.173.14.4363-4370.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed] [Google Scholar]
  9. Nelson M., McClelland M. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases. Nucleic Acids Res. 1989;17 (Suppl):r389–r415. doi: 10.1093/nar/17.suppl.r389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Price C., Bickle T. A. A possible role for DNA restriction in bacterial evolution. Microbiol Sci. 1986 Oct;3(10):296–299. [PubMed] [Google Scholar]
  11. Roberts R. J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2331–2365. doi: 10.1093/nar/18.suppl.2331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  13. Smith H. O., Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. doi: 10.1016/0022-2836(73)90152-6. [DOI] [PubMed] [Google Scholar]
  14. Solaiman D. K., Somkuti G. A. Isolation and characterization of a type II restriction endonuclease from Streptococcus thermophilus. FEMS Microbiol Lett. 1990 Feb;55(3):261–265. doi: 10.1016/0378-1097(90)90006-c. [DOI] [PubMed] [Google Scholar]
  15. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES