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. 1989 Jan;55(1):50–54. doi: 10.1128/aem.55.1.50-54.1989

Effects of Prechilling and Sequential Washing on Enumeration of Microorganisms from Refuse

M A Barlaz 1,†,*, D M Schaefer 1, R K Ham 1
PMCID: PMC184053  PMID: 16347834

Abstract

Techniques were evaluated for formation of a liquid inoculum from shredded municipal refuse, including chilling the refuse at 4°C prior to blending and multiple washing and blending cycles. The average count of cellulolytic bacteria from six different detachment treatments was 5.1 × 104 cells per g (dry weight) of refuse with a range of 0.7 × 104 to 12.7 × 104 cells per g (dry weight). The liquid obtained from blending the refuse in phosphate buffer followed by hand squeezing was the selected detachment procedure. The inoculum formation procedure was validated by the addition of ruminal cellulolytic bacteria to refuse and recovery of the cellulolytic bacteria by most-probable-number enumerations. The ratio of measured to expected cell counts among tests in which different volumes of ruminal fluid were added to refuse ranged from 2.7 to 14.4. There was no evidence of anaerobic cellulolytic fungi in a refuse sample.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akin D. E., Gordon G. L., Hogan J. P. Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur. Appl Environ Microbiol. 1983 Sep;46(3):738–748. doi: 10.1128/aem.46.3.738-748.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barlaz M. A., Schaefer D. M., Ham R. K. Bacterial population development and chemical characteristics of refuse decomposition in a simulated sanitary landfill. Appl Environ Microbiol. 1989 Jan;55(1):55–65. doi: 10.1128/aem.55.1.55-65.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bauchop T. Rumen anaerobic fungi of cattle and sheep. Appl Environ Microbiol. 1979 Jul;38(1):148–158. doi: 10.1128/aem.38.1.148-158.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dehority B. A., Grubb J. A. Effect of short-term chilling of rumen contents on viable bacterial numbers. Appl Environ Microbiol. 1980 Feb;39(2):376–381. doi: 10.1128/aem.39.2.376-381.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Leedle J. A., Bryant M. P., Hespell R. B. Diurnal variations in bacterial numbers and fluid parameters in ruminal contents of animals fed low- or high-forage diets. Appl Environ Microbiol. 1982 Aug;44(2):402–412. doi: 10.1128/aem.44.2.402-412.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Leedle J. A., Hespell R. B. Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations. Appl Environ Microbiol. 1980 Apr;39(4):709–719. doi: 10.1128/aem.39.4.709-719.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Orpin C. G. Studies on the rumen flagellate Neocallimastix frontalis. J Gen Microbiol. 1975 Dec;91(2):249–262. doi: 10.1099/00221287-91-2-249. [DOI] [PubMed] [Google Scholar]
  8. Windham W. R., Akin D. E. Rumen fungi and forage fiber degradation. Appl Environ Microbiol. 1984 Sep;48(3):473–476. doi: 10.1128/aem.48.3.473-476.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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