Abstract
Two mutants of Clostridium thermocellum were isolated after UV light mutagenesis. Mutant A1, selected as asporogenous, exhibited a fermentation pattern similar to that of the wild type. However, at pH 6.5, the mutant degraded 12% more cellulose than did the wild type, leading to enhanced ethanol production. Mutant 647, selected as ethanol tolerant, was able to grow in medium containing 4% ethanol. During the early stage of the exponential growth phase, ethanol was produced as the main product, up to a concentration of about 9 g/liter. After 3 days of culture, 48.3 g (89% of the initial amount) of degraded cellulose per liter was fermented into 12.7 g of ethanol per liter.
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Selected References
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- Emeruwa A. C., Hawirko R. Z., Halvorson H., Suzuki I. Comparison of butyric type of fermentation in sporogenic and asporogenic mutants of Clostridium botulinum. J Bacteriol. 1974 Oct;120(1):74–80. doi: 10.1128/jb.120.1.74-80.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsu E. J., Ordal Z. J. Comparative metabolism of vegetative and sporulating cultures of Clostridium thermosaccharolyticum. J Bacteriol. 1970 May;102(2):369–376. doi: 10.1128/jb.102.2.369-376.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones D. T., van der Westhuizen A., Long S., Allcock E. R., Reid S. J., Woods D. R. Solvent Production and Morphological Changes in Clostridium acetobutylicum. Appl Environ Microbiol. 1982 Jun;43(6):1434–1439. doi: 10.1128/aem.43.6.1434-1439.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tailliez P., Girard H., Millet J., Beguin P. Enhanced Cellulose Fermentation by an Asporogenous and Ethanol-Tolerant Mutant of Clostridium thermocellum. Appl Environ Microbiol. 1989 Jan;55(1):207–211. doi: 10.1128/aem.55.1.207-211.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]