Abstract
A 3,080-base-pair KpnI-HindIII DNA fragment from Bacillus sphaericus 2362 coding for 51- and 42-kilodalton mosquitocidal proteins was cloned into Bacillus subtilis DB104 by using the vector pUB18. In B. subtilis these proteins were not detected during vegetative growth but were expressed during sporulation at levels comparable to those found in B. sphaericus.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baumann L., Broadwell A. H., Baumann P. Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297. J Bacteriol. 1988 May;170(5):2045–2050. doi: 10.1128/jb.170.5.2045-2050.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumann P., Baumann L., Bowditch R. D., Broadwell A. H. Cloning of the gene for the larvicidal toxin of Bacillus sphaericus 2362: evidence for a family of related sequences. J Bacteriol. 1987 Sep;169(9):4061–4067. doi: 10.1128/jb.169.9.4061-4067.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumann P., Unterman B. M., Baumann L., Broadwell A. H., Abbene S. J., Bowditch R. D. Purification of the larvicidal toxin of Bacillus sphaericus and evidence for high-molecular-weight precursors. J Bacteriol. 1985 Aug;163(2):738–747. doi: 10.1128/jb.163.2.738-747.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broadwell A. H., Baumann P. Sporulation-associated activation of Bacillus sphaericus larvicide. Appl Environ Microbiol. 1986 Oct;52(4):758–764. doi: 10.1128/aem.52.4.758-764.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kawamura F., Doi R. H. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases. J Bacteriol. 1984 Oct;160(1):442–444. doi: 10.1128/jb.160.1.442-444.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tandeau de Marsac N., de la Torre F., Szulmajster J. Expression of the larvicidal gene of Bacillus sphaericus 1593M in the cyanobacterium Anacystis nidulans R2. Mol Gen Genet. 1987 Sep;209(2):396–398. doi: 10.1007/BF00329671. [DOI] [PubMed] [Google Scholar]
- Yousten A. A. Bacillus sphaericus: microbiological factors related to its potential as a mosquito larvicide. Adv Biotechnol Processes. 1984;3:315–343. [PubMed] [Google Scholar]
- Zaghloul T. I., Kawamura F., Doi R. H. Translational coupling in Bacillus subtilis of a heterologous Bacillus subtilis-Escherichia coli gene fusion. J Bacteriol. 1985 Nov;164(2):550–555. doi: 10.1128/jb.164.2.550-555.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]