Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1989 Jan;55(1):256–258. doi: 10.1128/aem.55.1.256-258.1989

Cyanase-mediated utilization of cyanate in Pseudomonas fluorescens NCIB 11764.

D A Kunz 1, O Nagappan 1
PMCID: PMC184089  PMID: 2495763

Abstract

Pseudomonas fluorescens NCIB 11764 was capable of utilizing cyanate (OCN-) as a sole nitrogen source for growth. Crude cell extracts from cells grown on cyanate, but not on ammonium sulfate, were induced for an enzyme catalyzing cyanate conversion to ammonia. Enzymatic activity was shown to be bicarbonate dependent and specific for cyanate as a substrate, suggesting that cyanate utilization in this organism is facilitated by an enzyme resembling cyanase (cyanate amidohydrolase; EC 3.5.5.3), as described previously in Escherichia coli and Flavobacterium sp.

Full text

PDF
256

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN C. M., Jr, JONES M. E. DECOMPOSITION OF CARBAMYLPHOSPHATE IN AQUEOUS SOLUTIONS. Biochemistry. 1964 Sep;3:1238–1247. doi: 10.1021/bi00897a010. [DOI] [PubMed] [Google Scholar]
  2. Anderson P. M., Little R. M. Kinetic properties of cyanase. Biochemistry. 1986 Apr 8;25(7):1621–1626. doi: 10.1021/bi00355a026. [DOI] [PubMed] [Google Scholar]
  3. Anderson P. M. Purification and properties of the inducible enzyme cyanase. Biochemistry. 1980 Jun 24;19(13):2882–2888. doi: 10.1021/bi00554a010. [DOI] [PubMed] [Google Scholar]
  4. Guilloton M., Hargreaves A. B. Hydrolyse du cyanate par une bactérie du genre Flavobacterium. C R Acad Sci Hebd Seances Acad Sci D. 1972 Oct 16;275(16):1827–1830. [PubMed] [Google Scholar]
  5. Guilloton M., Karst F. A spectrophotometric determination of cyanate using reaction with 2-aminobenzoic acid. Anal Biochem. 1985 Sep;149(2):291–295. doi: 10.1016/0003-2697(85)90572-x. [DOI] [PubMed] [Google Scholar]
  6. Guilloton M., Karst F. Cyanate specifically inhibits arginine biosynthesis in Escherichia coli K12: a case of by-product inhibition? J Gen Microbiol. 1987 Mar;133(3):655–665. doi: 10.1099/00221287-133-3-655. [DOI] [PubMed] [Google Scholar]
  7. Guilloton M., Karst F. Isolation and characterization of Escherichia coli mutants lacking inducible cyanase. J Gen Microbiol. 1987 Mar;133(3):645–653. doi: 10.1099/00221287-133-3-645. [DOI] [PubMed] [Google Scholar]
  8. Hagel P., Gerding J. J., Fieggen W., Bloemendal H. Cyanate formation in solutions of urea. I. Calculation of cyanate concentrations at different temperature and pH. Biochim Biophys Acta. 1971 Sep 28;243(3):366–373. doi: 10.1016/0005-2795(71)90003-1. [DOI] [PubMed] [Google Scholar]
  9. Harris R., Knowles C. J. Isolation and growth of a Pseudomonas species that utilizes cyanide as a source of nitrogen. J Gen Microbiol. 1983 Apr;129(4):1005–1011. doi: 10.1099/00221287-129-4-1005. [DOI] [PubMed] [Google Scholar]
  10. Kunz D. A., Ribbons D. W., Chapman P. J. Metabolism of allylglycine and cis-crotylglycine by Pseudomonas putida (arvilla) mt-2 harboring a TOL plasmid. J Bacteriol. 1981 Oct;148(1):72–82. doi: 10.1128/jb.148.1.72-82.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Sung Y. C., Anderson P. M., Fuchs J. A. Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase. J Bacteriol. 1987 Nov;169(11):5224–5230. doi: 10.1128/jb.169.11.5224-5230.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sung Y. C., Parsell D., Anderson P. M., Fuchs J. A. Identification, mapping, and cloning of the gene encoding cyanase in Escherichia coli K-12. J Bacteriol. 1987 Jun;169(6):2639–2642. doi: 10.1128/jb.169.6.2639-2642.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. TAUSSIG A. The synthesis of the induced enzyme, ''cyanase'', in E. coli. Biochim Biophys Acta. 1960 Nov 18;44:510–519. doi: 10.1016/0006-3002(60)91605-x. [DOI] [PubMed] [Google Scholar]
  16. Taussig A. Some properties of the induced enzyme cyanase. Can J Biochem. 1965 Jul;43(7):1063–1069. doi: 10.1139/o65-119. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES