Abstract
We compared the replication of the gypsy moth (Lymantria dispar) nuclear polyhedrosis virus in two new cell lines, from embryos and fat body of L. dispar, and in a previously available ovarian cell line. Three virus isolates (the Hamden strain [LDP-67] used commercially as GYPCHEK, a plaque-purified clone of Hamden [5-7d], and an isolate from Abington, Mass. [Ab]) were each tested on the three cell lines. The fat-body-derived cell line proved best in terms of occlusion body production for all three virus strains, with the highest yield produced by the Abington strain. On the basis of these results, we conclude that a more efficient in vitro production of gypsy moth virus can be obtained by using the fat body cell line in conjunction with the Abington strain of the virus.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goodwin R. H., Tompkins G. J., McCawley P. Gypsy moth cell lines divergent in viral susceptibility. I. Culture and identification. In Vitro. 1978 Jun;14(6):485–494. doi: 10.1007/BF02616088. [DOI] [PubMed] [Google Scholar]