Abstract
Streptomyces viridosporus T7A and S. badius 252 were grown in 1 to 2% (wt/vol) slurry cultures with mineral salts solution containing 0.6% yeast extract and 100/200 mesh ground and extracted corn lignocellulose at 37°C. Enzyme activities rapidly increased in the first 3 to 4 days and then declined and remained at a relatively constant level. Concentrations of endoglucanase and xylanase produced by S. badius were lower than those produced by S. viridosporus. However, the lignin-peroxidase peak concentration was threefold higher than with S. viridosporus and was obtained at 9 to 10 days of incubation. By polyacrylamide gel analysis, it was determined that peroxidases from both species consisted of four enzymes, with only one, the lignin peroxidase, having high activity. A culture pH of 8.5 was preferable for lignocellulose degradation by S. badius.
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- Borgmeyer J. R., Crawford D. L. Production and Characterization of Polymeric Lignin Degradation Intermediates from Two Different Streptomyces spp. Appl Environ Microbiol. 1985 Feb;49(2):273–278. doi: 10.1128/aem.49.2.273-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crawford D. L., Pometto A. L., Crawford R. L. Lignin Degradation by Streptomyces viridosporus: Isolation and Characterization of a New Polymeric Lignin Degradation Intermediate. Appl Environ Microbiol. 1983 Mar;45(3):898–904. doi: 10.1128/aem.45.3.898-904.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pometto A. L., Crawford D. L. Effects of pH on Lignin and Cellulose Degradation by Streptomyces viridosporus. Appl Environ Microbiol. 1986 Aug;52(2):246–250. doi: 10.1128/aem.52.2.246-250.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pridham T. G., Gottlieb D. The Utilization of Carbon Compounds by Some Actinomycetales as an Aid for Species Determination. J Bacteriol. 1948 Jul;56(1):107–114. doi: 10.1128/jb.56.1.107-114.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramachandra M., Crawford D. L., Hertel G. Characterization of an extracellular lignin peroxidase of the lignocellulolytic actinomycete Streptomyces viridosporus. Appl Environ Microbiol. 1988 Dec;54(12):3057–3063. doi: 10.1128/aem.54.12.3057-3063.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramachandra M., Crawford D. L., Pometto A. L. Extracellular Enzyme Activities during Lignocellulose Degradation by Streptomyces spp.: A Comparative Study of Wild-Type and Genetically Manipulated Strains. Appl Environ Microbiol. 1987 Dec;53(12):2754–2760. doi: 10.1128/aem.53.12.2754-2760.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]