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. 1989 May;55(5):1262–1266. doi: 10.1128/aem.55.5.1262-1266.1989

Temperature Compensation in Methanosarcina barkeri by Modulation of Hydrogen and Acetate Affinity

Peter Westermann 1,†, Birgitte K Ahring 1,‡,*, Robert A Mah 1
PMCID: PMC184287  PMID: 16347915

Abstract

The affinity of Methanosarcina barkeri 227 for acetate and hydrogen at different incubation temperatures was investigated. Increasing the temperature from 20 to 37°C resulted in a 4.5-fold increase in Km for acetate and a 4.8-fold increase for hydrogen. The corresponding increase in Vmax for acetate was 8.3-fold (5.4-fold for hydrogen). This response implied a decrease in the temperature coefficient (Q10) and hence a decrease in the temperature dependency as a function of decreasing substrate concentration.

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Selected References

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