Abstract
A method is described for the purification of Bacillus thuringiensis protein crystals by Ludox gradient centrifugation. This method is simple, inexpensive, fast, and efficient compared with other techniques. It has been successfully used to purify and characterize the protein crystals from several B. thuringiensis strains.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ang B. J., Nickerson K. W. Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation. Appl Environ Microbiol. 1978 Oct;36(4):625–626. doi: 10.1128/aem.36.4.625-626.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodman N. S., Gottfried R. J., Rogoff M. H. Biphasic system for separation of spores and crystals of Bacillus thuringiensis. J Bacteriol. 1967 Aug;94(2):485–485. doi: 10.1128/jb.94.2.485-.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haider M. Z., Ward E. S., Ellar D. J. Cloning and heterologous expression of an insecticidal delta-endotoxin gene from Bacillus thuringiensis var. aizawai IC1 toxic to both lepidoptera and diptera. Gene. 1987;52(2-3):285–290. doi: 10.1016/0378-1119(87)90055-2. [DOI] [PubMed] [Google Scholar]
- Sharpe E. S., Nickerson K. W., Bulla L. A., Jr, Aronson J. N. Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents. Appl Microbiol. 1975 Dec;30(6):1052–1053. doi: 10.1128/am.30.6.1052-1053.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]