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. 1989 May;55(5):1317–1319. doi: 10.1128/aem.55.5.1317-1319.1989

Synthetic Medium for Acetobacter xylinum That Can Be Used for Isolation of Auxotrophic Mutants and Study of Cellulose Biosynthesis

E R Forng 1,†, S M Anderson 1, R E Cannon 1,*
PMCID: PMC184302  PMID: 16347923

Abstract

Acetobacter xylinum is a bacterium that can synthesize cellulose when grown in an undefined medium containing glucose. We developed a defined minimal medium for A. xylinum that contains 1% glucose, 0.1% NH4Cl, 0.115% citric acid, 0.33% Na2HPO4, 0.01% KCl, 0.025% MgSO4. 7H2O, and 7.5 mg of nicotinamide per liter which both allows cellulose synthesis and can be used to isolate a variety of auxotrophic mutants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Cohen R., Benziman M., Delmer D. Solubilization of the UDP-glucose:1,4-beta-D-glucan 4-beta-D-glucosyltransferase (cellulose synthase) from Acetobacter xylinum. A comparison of regulatory properties with those of the membrane-bound form of the enzyme. J Biol Chem. 1983 Apr 10;258(7):4419–4423. [PubMed] [Google Scholar]
  2. DUDMAN W. F. Cellulose production by Acetobacter actigenum in defined medium. J Gen Microbiol. 1959 Oct;21:327–337. doi: 10.1099/00221287-21-2-327. [DOI] [PubMed] [Google Scholar]
  3. GLASER L. The synthesis of cellulose in cell-free extracts of Acetobacter xylinum. J Biol Chem. 1958 Jun;232(2):627–636. [PubMed] [Google Scholar]
  4. HESTRIN S., SCHRAMM M. Synthesis of cellulose by Acetobacter xylinum. II. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. Biochem J. 1954 Oct;58(2):345–352. doi: 10.1042/bj0580345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Montezinos D., Brown R. M., Jr Cell wall biogenesis in Oocystis: experimental alteration of microfibril assembly and orientation. Cytobios. 1978;23(90):119–139. [PubMed] [Google Scholar]
  6. Swissa M., Aloni Y., Weinhouse H., Benizman M. Intermediatry steps in Acetobacter xylinum cellulose synthesis: studies with whole cells and cell-free preparations of the wild type and a celluloseless mutant. J Bacteriol. 1980 Sep;143(3):1142–1150. doi: 10.1128/jb.143.3.1142-1150.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. TEPPER B. S., LITSKY W. The nutritional requirements of Acetobacter. III. Amino acid requirements of Acetobacter xylinum. Growth. 1953 Dec;17(4):193–199. [PubMed] [Google Scholar]

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