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. 1990 Apr;56(4):943–948. doi: 10.1128/aem.56.4.943-948.1990

Utilization of Glycerol as a Hydrogen Acceptor by Lactobacillus reuteri: Purification of 1,3-Propanediol:NAD+ Oxidoreductase †

Todd L Talarico 1, Lars T Axelsson 1, James Novotny 1, Mitra Fiuzat 1, Walter J Dobrogosz 1,*
PMCID: PMC184326  PMID: 16348177

Abstract

Lactobacillus reuteri utilizes exogenously added glycerol as a hydrogen acceptor during carbohydrate fermentations, resulting in higher growth rates and cell yields than those obtained during growth on carbohydrates alone. Glycerol is first converted to 3-hydroxypropionaldehyde by a coenzyme B12-dependent glycerol dehydratase and then reduced to 1,3-propanediol by an NAD+ -dependent oxidoreductase. The latter enzyme was purified and determined to have a molecular weight of 180,000; it is predicted to exist as a tetramer of identical 42,000-molecular-weight subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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