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. 1990 Apr;56(4):1195–1197. doi: 10.1128/aem.56.4.1195-1197.1990

Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri†

Todd L Talarico 1, Walter J Dobrogosz 1,*
PMCID: PMC184372  PMID: 16348166

Abstract

A coenzyme B12-dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. Km values for substrates and coenzyme B12 were in the millimolar and the submicromolar range, respectively.

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Selected References

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