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. 1990 Jun;56(6):1541–1546. doi: 10.1128/aem.56.6.1541-1546.1990

Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection.

H A Lee 1, G M Wyatt 1, S Bramham 1, M R Morgan 1
PMCID: PMC184468  PMID: 2200337

Abstract

A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to the enzyme-linked immunosorbent assay, low numbers of cells in food (10 cells 25 g-1) were detected in 19 h. Non-Salmonella competing organisms did not interfere with detection of S. typhimurium even when present in the ratio of 10(6):1 (non-Salmonella/Salmonella spp.). The assay shows the feasibility of rapid, 1-day testing for Salmonella spp. with antibody technology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Challacombe S. J., Biggerstaff M., Greenall C., Kemeny D. M. ELISA detection of human IgG subclass antibodies to Streptococcus mutans. J Immunol Methods. 1986 Feb 27;87(1):95–102. doi: 10.1016/0022-1759(86)90348-0. [DOI] [PubMed] [Google Scholar]
  2. Flowers R. S., Eckner K., Gabis D. A., Robison B. J., Mattingly J. A., Silliker J. H. Enzyme immunoassay for detection of Salmonella in foods: collaborative study. J Assoc Off Anal Chem. 1986 Sep-Oct;69(5):786–798. [PubMed] [Google Scholar]
  3. Flowers R. S., Klatt M. J., Keelan S. L. Visual immunoassay for detection of Salmonella in foods: collaborative study. J Assoc Off Anal Chem. 1988 Sep-Oct;71(5):973–980. [PubMed] [Google Scholar]
  4. Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
  5. Hadfield S. G., Lane A., McIllmurray M. B. A novel coloured latex test for the detection and identification of more than one antigen. J Immunol Methods. 1987 Mar 12;97(2):153–158. doi: 10.1016/0022-1759(87)90454-6. [DOI] [PubMed] [Google Scholar]
  6. Harford J. P. An evaluation of a commercially available enzyme immunoassay test for the rapid detection of salmonellae in food and environmental samples. Epidemiol Infect. 1987 Aug;99(1):127–136. doi: 10.1017/s0950268800066930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ibrahim G. F., Fleet G. H., Lyons M. J., Walker R. A. Method for the isolation of highly purified Salmonella flagellins. J Clin Microbiol. 1985 Dec;22(6):1040–1044. doi: 10.1128/jcm.22.6.1040-1044.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KENNER B. A., ROCKWOOD S. W., KABLER P. W. Isolation of members of the genus Salmonella by membrane filter procedures. Appl Microbiol. 1957 Sep;5(5):305–307. doi: 10.1128/am.5.5.305-307.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  10. Krysinski E. P., Heimsch R. C. Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977 Apr;33(4):947–954. doi: 10.1128/aem.33.4.947-954.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Mattingly J. A. An enzyme immunoassay for the detection of all Salmonella using a combination of a myeloma protein and a hybridoma antibody. J Immunol Methods. 1984 Oct 12;73(1):147–156. doi: 10.1016/0022-1759(84)90040-1. [DOI] [PubMed] [Google Scholar]
  13. Robison B. J., Pretzman C. I., Mattingly J. A. Enzyme immunoassay in which a myeloma protein is used for detection of salmonellae. Appl Environ Microbiol. 1983 Jun;45(6):1816–1821. doi: 10.1128/aem.45.6.1816-1821.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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