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. 1990 Jun;56(6):1971–1973. doi: 10.1128/aem.56.6.1971-1973.1990

Purification and Properties of Catechol 1,2-Dioxygenase from Rhizobium leguminosarum biovar viceae USDA 2370

Yung Pin Chen 1, Charles R Lovell 1,*
PMCID: PMC184541  PMID: 16348234

Abstract

The catechol 1,2-dioxygenase of Rhizobium leguminosarum biovar viceae USDA 2370 was purified 296-fold, yielding a homogeneous preparation with a specific activity of 51.1 U mg of protein-1. The molecular weight of the native protein was 70,000, with two identical subunits of 34,500 and 1 g-atom of iron per mol. The optimum pH for catalytic activity was 9.0 to 9.5.

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Selected References

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  1. Brown C. M., Dilworth M. J. Ammonia assimilation by rhizobium cultures and bacteroids. J Gen Microbiol. 1975 Jan;86(1):39–48. doi: 10.1099/00221287-86-1-39. [DOI] [PubMed] [Google Scholar]
  2. HAYAISHI O., KATAGIRI M., ROTHBERG S. Studies on oxygenases; pyrocatechase. J Biol Chem. 1957 Dec;229(2):905–920. [PubMed] [Google Scholar]
  3. Kojima Y., Fujisawa H., Nakazawa A., Nakazawa T., Kanetsuna F., Taniuchi H., Nozaki M., Hayaishi O. Studies on pyrocatechase. I. Purification and spectral properties. J Biol Chem. 1967 Jul 25;242(14):3270–3278. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. NAKAGAWA H., INOUE H., TAKEDA Y. CHARACTERISTICS OF CATECHOL OXYGENASE FROM BREVIBACTERIUM FUSCUM. J Biochem. 1963 Jul;54:65–74. doi: 10.1093/oxfordjournals.jbchem.a127748. [DOI] [PubMed] [Google Scholar]
  7. Nakai C., Nakazawa T., Nozaki M. Purification and properties of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas putida mt-2 in comparison with that from Pseudomonas arvilla C-1. Arch Biochem Biophys. 1988 Dec;267(2):701–713. doi: 10.1016/0003-9861(88)90079-3. [DOI] [PubMed] [Google Scholar]
  8. Ono K., Nozaki M., Hayaishi O. Purification and some properties of protocatechuate 4,5-dioxygenase. Biochim Biophys Acta. 1970 Nov 11;220(2):224–238. doi: 10.1016/0005-2744(70)90008-2. [DOI] [PubMed] [Google Scholar]
  9. Stanier R. Y., Ornston L. N. The beta-ketoadipate pathway. Adv Microb Physiol. 1973;9(0):89–151. [PubMed] [Google Scholar]

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