Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jul;56(7):2114–2119. doi: 10.1128/aem.56.7.2114-2119.1990

Effects of cyclohexane, an industrial solvent, on the yeast Saccharomyces cerevisiae and on isolated yeast mitochondria.

S Uribe 1, P Rangel 1, G Espínola 1, G Aguirre 1
PMCID: PMC184569  PMID: 2202257

Abstract

Little information on the effects of cyclohexane at the cellular or subcellular level is available. In Saccharomyces cerevisiae, cyclohexane inhibited respiration and diverse energy-dependent processes. In mitochondria isolated from S. cerevisiae, oxygen uptake and ATP synthesis were inhibited, although ATPase activity was not affected. Cyclohexane effects were similar to those reported for beta-pinene and limonene, suggesting that the cyclohexane ring in these monoterpenes may be a determinant for their biological activities.

Full text

PDF
2114

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akerman K. E., Wikström M. K. Safranine as a probe of the mitochondrial membrane potential. FEBS Lett. 1976 Oct 1;68(2):191–197. doi: 10.1016/0014-5793(76)80434-6. [DOI] [PubMed] [Google Scholar]
  2. Mutti A., Lucertini S., Falzoi M., Cavatorta A., Franchini I. Organic solvents and chronic glomerulonephritis: a cross-sectional study with negative findings for aliphatic and alicyclic C5-C7 hydrocarbons. J Appl Toxicol. 1981 Aug;1(4):224–226. doi: 10.1002/jat.2550010408. [DOI] [PubMed] [Google Scholar]
  3. Perbellini L., Brugnone F. Lung uptake and metabolism of cyclohexane in shoe factory workers. Int Arch Occup Environ Health. 1980;45(3):261–269. doi: 10.1007/BF00380789. [DOI] [PubMed] [Google Scholar]
  4. Peña A., Piña M. Z., Escamilla E., Piña E. A novel method for the rapid preparation of coupled yeast mitochondria. FEBS Lett. 1977 Aug 1;80(1):209–213. doi: 10.1016/0014-5793(77)80441-9. [DOI] [PubMed] [Google Scholar]
  5. Peña A., Uribe S., Pardo J. P., Borbolla M. The use of a cyanine dye in measuring membrane potential in yeast. Arch Biochem Biophys. 1984 May 15;231(1):217–225. doi: 10.1016/0003-9861(84)90381-3. [DOI] [PubMed] [Google Scholar]
  6. Pick U., Weiss M., Rottenberg H. Anomalous uncoupling of photophosphorylation by palmitic acid and by gramicidin D. Biochemistry. 1987 Dec 15;26(25):8295–8302. doi: 10.1021/bi00399a041. [DOI] [PubMed] [Google Scholar]
  7. Rottenberg H., Hashimoto K. Fatty acid uncoupling of oxidative phosphorylation in rat liver mitochondria. Biochemistry. 1986 Apr 8;25(7):1747–1755. doi: 10.1021/bi00355a045. [DOI] [PubMed] [Google Scholar]
  8. Savolainen H., Pfäffli P. Burden and dose-related neurochemical effects of intermittent cyclohexane vapour inhalation in rats. Toxicol Lett. 1980 Nov;7(1):17–22. doi: 10.1016/0378-4274(80)90079-x. [DOI] [PubMed] [Google Scholar]
  9. Sumner J. B. A METHOD FOR THE COLORIMETRIC DETERMINATION OF PHOSPHORUS. Science. 1944 Nov 3;100(2601):413–414. doi: 10.1126/science.100.2601.413. [DOI] [PubMed] [Google Scholar]
  10. Tham R., Bunnfors I., Eriksson B., Larsby B., Lindgren S., Odkvist L. M. Vestibulo-ocular disturbances in rats exposed to organic solvents. Acta Pharmacol Toxicol (Copenh) 1984 Jan;54(1):58–63. doi: 10.1111/j.1600-0773.1984.tb01895.x. [DOI] [PubMed] [Google Scholar]
  11. Uribe S., Ramirez J., Peña A. Effects of beta-pinene on yeast membrane functions. J Bacteriol. 1985 Mar;161(3):1195–1200. doi: 10.1128/jb.161.3.1195-1200.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. de KLOET S., van WERMESKERKEN R., KONINGSBERGER V. V. Studies on protein synthesis by protoplasts of Saccharomyces carlsbergensis. I. The effect of ribonuclease on protein synthesis. Biochim Biophys Acta. 1961 Feb 12;47:138–143. doi: 10.1016/0006-3002(61)90838-1. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES