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. 1990 Jul;56(7):2200–2205. doi: 10.1128/aem.56.7.2200-2205.1990

Thermal ecology of Naegleria fowleri from a power plant cooling reservoir.

H W Huizinga 1, G L McLaughlin 1
PMCID: PMC184583  PMID: 1975164

Abstract

The pathogenic, free-living amoeba Naegleria fowleri is the causative agent of human primary amebic meningoencephalitis. N. fowleri has been isolated from thermally elevated aquatic environments worldwide, but temperature factors associated with occurrence of the amoeba remain undefined. In this study, a newly created cooling reservoir (Clinton Lake, Illinois) was surveyed for Naegleria spp. before and after thermal additions from a nuclear power plant. Water and sediment samples were collected from heated and unheated arms of the reservoir and analyzed for the presence of thermophilic Naegleria spp. and pathogenic N. fowleri. Amoebae were identified by morphology, in vitro cultivation, temperature tolerance, mouse pathogenicity assay, and DNA restriction fragment length analysis. N. fowleri was isolated from the thermally elevated arm but not from the ambient-temperature arm of the reservoir. The probability of isolating thermophilic Naegleria and pathogenic N. fowleri increased significantly with temperature. Repetitive DNA restriction fragment profiles of the N. fowleri Clinton Lake isolates and a known N. fowleri strain of human origin were homogeneous.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cable B. L., John D. T. Conditions for maximum enflagellation in Naegleria fowleri. J Protozool. 1986 Nov;33(4):467–472. doi: 10.1111/j.1550-7408.1986.tb05643.x. [DOI] [PubMed] [Google Scholar]
  2. De Jonckheere J. F. Characterization of Naegleria species by restriction endonuclease digestion of whole-cell DNA. Mol Biochem Parasitol. 1987 May;24(1):55–66. doi: 10.1016/0166-6851(87)90115-0. [DOI] [PubMed] [Google Scholar]
  3. De Jonckheere J. F. Geographic origin and spread of pathogenic Naegleria fowleri deduced from restriction enzyme patterns of repeated DNA. Biosystems. 1988;21(3-4):269–275. doi: 10.1016/0303-2647(88)90022-6. [DOI] [PubMed] [Google Scholar]
  4. Griffin J. L. Temperature tolerance of pathogenic and nonpathogenic free-living amoebas. Science. 1972 Nov 24;178(4063):869–870. doi: 10.1126/science.178.4063.869. [DOI] [PubMed] [Google Scholar]
  5. Griffin J. L. The pathogenic amoeboflagellate Naegleria fowleri: environmental isolations, competitors, ecologic interactions, and the flagellate-empty habitat hypothesis. J Protozool. 1983 May;30(2):403–409. doi: 10.1111/j.1550-7408.1983.tb02939.x. [DOI] [PubMed] [Google Scholar]
  6. Josephson S. L., Weik R. R., John D. T. Concanavalin A-induced agglutination of Naegleria. Am J Trop Med Hyg. 1977 Sep;26(5 Pt 1):856–858. doi: 10.4269/ajtmh.1977.26.856. [DOI] [PubMed] [Google Scholar]
  7. McLaughlin G. L., Brandt F. H., Visvesvara G. S. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amebae. J Clin Microbiol. 1988 Sep;26(9):1655–1658. doi: 10.1128/jcm.26.9.1655-1658.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stevens A. R., Tyndall R. L., Coutant C. C., Willaert E. Isolation of the etiological agent of primary amoebic meningoencephalitis from artifically heated waters. Appl Environ Microbiol. 1977 Dec;34(6):701–705. doi: 10.1128/aem.34.6.701-705.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sykora J. L., Keleti G., Martinez A. J. Occurrence and pathogenicity of Naegleria fowleri in artificially heated waters. Appl Environ Microbiol. 1983 Mar;45(3):974–979. doi: 10.1128/aem.45.3.974-979.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tyndall R. L., Ironside K. S., Metler P. L., Tan E. L., Hazen T. C., Fliermans C. B. Effect of thermal additions on the density and distribution of thermophilic amoebae and pathogenic Naegleria fowleri in a newly created cooling lake. Appl Environ Microbiol. 1989 Mar;55(3):722–732. doi: 10.1128/aem.55.3.722-732.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weik R. R., John D. T. Agitated mass cultivation of Naegleria fowleri. J Parasitol. 1977 Oct;63(5):868–871. [PubMed] [Google Scholar]
  12. Wellings F. M., Amuso P. T., Chang S. L., Lewis A. L. Isolation and identification of pathogenic Naegleria from Florida lakes. Appl Environ Microbiol. 1977 Dec;34(6):661–667. doi: 10.1128/aem.34.6.661-667.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Jonckheere J., Voorde H. The distribution of Naegleria fowleri in man-made thermal waters. Am J Trop Med Hyg. 1977 Jan;26(1):10–15. doi: 10.4269/ajtmh.1977.26.10. [DOI] [PubMed] [Google Scholar]

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