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. 1990 Jul;56(7):2206–2212. doi: 10.1128/aem.56.7.2206-2212.1990

New Field Isolates of Rhizobium leguminosarum Biovar Viciae That Nodulate the Primitive Pea Cultivar Afghanistan in Addition to Modern Cultivars

S-W Ma 1, V N Iyer 1,*
PMCID: PMC184584  PMID: 16348240

Abstract

A collection of 13 field isolates of Rhizobium leguminosarum bv. viciae that have the ability to nodulate the roots of current North American cultivars of peas as well as a “primitive” cultivar, Afghanistan, was examined. These isolates originated in diverse geographical regions of the world, which indicates that this phenotype is not restricted to isolates from any one region. When subclones of the nodulation region from one plasmid were used to examine EcoRI-fragment-length polymorphisms in this collection of strains as well as in a collection comprising strains that do not nodulate the primitive cultivar, polymorphism was found in both collections. With one exception, RisφA6, all strains that nodulated cv. Afghanistan pea contained a region called nodX as an extension to the nodA BCIJ operon that has been observed in all R. leguminosarum bv. viciae strains, including those that do not nodulate cv. Afghanistan pea. RisφA6 was also the only strain in which nodulating ability could not be associated with a conjugative plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S. The ecology of transferable drug resistance in the enterobacteria. Annu Rev Microbiol. 1968;22:131–180. doi: 10.1146/annurev.mi.22.100168.001023. [DOI] [PubMed] [Google Scholar]
  2. Beringer J. E. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974 Sep;84(1):188–198. doi: 10.1099/00221287-84-1-188. [DOI] [PubMed] [Google Scholar]
  3. Davies R. R., Miller M., Turner S. J., Watson M., McGill A., Orskov H., Alberti K. G., Johnston D. G. Effects of somatostatin analogue SMS 201-995 in non-insulin-dependent diabetes. Clin Endocrinol (Oxf) 1986 Dec;25(6):739–747. doi: 10.1111/j.1365-2265.1986.tb03630.x. [DOI] [PubMed] [Google Scholar]
  4. Davis E. O., Evans I. J., Johnston A. W. Identification of nodX, a gene that allows Rhizobium leguminosarum biovar viciae strain TOM to nodulate Afghanistan peas. Mol Gen Genet. 1988 Jun;212(3):531–535. doi: 10.1007/BF00330860. [DOI] [PubMed] [Google Scholar]
  5. Honma M. A., Ausubel F. M. Rhizobium meliloti has three functional copies of the nodD symbiotic regulatory gene. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8558–8562. doi: 10.1073/pnas.84.23.8558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Horvath B., Kondorosi E., John M., Schmidt J., Török I., Györgypal Z., Barabas I., Wieneke U., Schell J., Kondorosi A. Organization, structure and symbiotic function of Rhizobium meliloti nodulation genes determining host specificity for alfalfa. Cell. 1986 Aug 1;46(3):335–343. doi: 10.1016/0092-8674(86)90654-9. [DOI] [PubMed] [Google Scholar]
  7. Long S. R. Rhizobium-legume nodulation: life together in the underground. Cell. 1989 Jan 27;56(2):203–214. doi: 10.1016/0092-8674(89)90893-3. [DOI] [PubMed] [Google Scholar]
  8. Love H. D., Jr, Allen-Nash A., Zhao Q. A., Bannon G. A. mRNA stability plays a major role in regulating the temperature-specific expression of a Tetrahymena thermophila surface protein. Mol Cell Biol. 1988 Jan;8(1):427–432. doi: 10.1128/mcb.8.1.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Selvaraj G., Iyer V. N. Suicide plasmid vehicles for insertion mutagenesis in Rhizobium meliloti and related bacteria. J Bacteriol. 1983 Dec;156(3):1292–1300. doi: 10.1128/jb.156.3.1292-1300.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith G. E., Summers M. D. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Anal Biochem. 1980 Nov 15;109(1):123–129. doi: 10.1016/0003-2697(80)90019-6. [DOI] [PubMed] [Google Scholar]
  11. Soberón-Chávez G., Nájera R., Olivera H., Segovia L. Genetic rearrangements of a Rhizobium phaseoli symbiotic plasmid. J Bacteriol. 1986 Aug;167(2):487–491. doi: 10.1128/jb.167.2.487-491.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zohary D., Hopf M. Domestication of Pulses in the Old World: Legumes were companions of wheat and barley when agriculture began in the Near East. Science. 1973 Nov 30;182(4115):887–894. doi: 10.1126/science.182.4115.887. [DOI] [PubMed] [Google Scholar]

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