Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jul;56(7):2259–2261. doi: 10.1128/aem.56.7.2259-2261.1990

Conditions for growing Mycoplasma canadense and Mycoplasma verecundum in a serum-free medium.

G Muñoz 1, P Sotomayor 1
PMCID: PMC184595  PMID: 2202260

Abstract

Mycoplasma canadense and Mycoplasma verecundum were cultured in a serum-free medium containing bovine serum albumin, cholesterol, oleic acid, and palmitic acid in order to avoid the addition of horse serum. Growth was detected by measurement of A640 and by colony formation. The level of growth attained in this medium was less than that obtained in the horse serum-supplemented media, but colonies retained their distinctive morphology.

Full text

PDF
2259

Images in this article

2260Image
on p.2260
2261Image
on p.2261

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clejan S., Bittman R. Distribution and movement of sterols with different side chain structures between the two leaflets of the membrane bilayer of mycoplasma cells. J Biol Chem. 1984 Jan 10;259(1):449–455. [PubMed] [Google Scholar]
  2. Clejan S., Bittman R. Kinetics of cholesterol and phospholipid exchange between Mycoplasma gallisepticum cells and lipid vesicles. Alterations in membrane cholesterol and protein content. J Biol Chem. 1984 Jan 10;259(1):441–448. [PubMed] [Google Scholar]
  3. Cluss R. G., Johnson J. K., Somerson N. L. Liposomes replace serum for cultivation of fermenting mycoplasmas. Appl Environ Microbiol. 1983 Aug;46(2):370–374. doi: 10.1128/aem.46.2.370-374.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gourlay R. N., Leach R. H., Howard C. J. Mycoplasma verecundum, a new species isolated from bovine eyes. J Gen Microbiol. 1974 Apr;81(2):475–484. doi: 10.1099/00221287-81-2-475. [DOI] [PubMed] [Google Scholar]
  5. Harasawa R., Mizusawa H., Koshimizu K. A reliable and sensitive method for detecting mycoplasmas in cell cultures. Microbiol Immunol. 1986;30(9):919–921. doi: 10.1111/j.1348-0421.1986.tb03020.x. [DOI] [PubMed] [Google Scholar]
  6. Le Grimellec C., Cardinal J., Giocondi M. C., Carrière S. Control of membrane lipids in Mycoplasma gallisepticum: effect on lipid order. J Bacteriol. 1981 Apr;146(1):155–162. doi: 10.1128/jb.146.1.155-162.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. RAZIN S., OLIVER O. Morphogenesis of Mycoplasma and bacterial L-form colonies. J Gen Microbiol. 1961 Feb;24:225–237. doi: 10.1099/00221287-24-2-225. [DOI] [PubMed] [Google Scholar]
  8. Razin S. Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol Rev. 1985 Dec;49(4):419–455. doi: 10.1128/mr.49.4.419-455.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Razin S., Tully J. G. Cholesterol requirement of mycoplasmas. J Bacteriol. 1970 May;102(2):306–310. doi: 10.1128/jb.102.2.306-310.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rodwell A. W. The supply of cholesterol and fatty acids for the growth of mycoplasmas. J Gen Microbiol. 1969 Sep;58(1):29–37. doi: 10.1099/00221287-58-1-29. [DOI] [PubMed] [Google Scholar]
  11. Rottem S., Greenberg A. S. Changes in composition, biosynthesis, and physical state of membrane lipids occurring upon aging of Mycoplasma hominis cultures. J Bacteriol. 1975 Feb;121(2):631–639. doi: 10.1128/jb.121.2.631-639.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rottem S. Membrane lipids of mycoplasmas. Biochim Biophys Acta. 1980 May 27;604(1):65–90. doi: 10.1016/0005-2736(80)90585-4. [DOI] [PubMed] [Google Scholar]
  13. Sasaki T., Shintani M., Kihara K. Comparison of the efficacy of egg yolk extract and horse serum for growth of Mycoplasma pneumoniae. Microbiol Immunol. 1985;29(6):499–507. doi: 10.1111/j.1348-0421.1985.tb00852.x. [DOI] [PubMed] [Google Scholar]
  14. Washburn L. R., Hughes J. H., Somerson N. L. Mycoplasma growth factors in bovine serum fraction. J Bacteriol. 1978 Sep;135(3):818–827. doi: 10.1128/jb.135.3.818-827.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES