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. 1990 Jul;56(7):2265–2267. doi: 10.1128/aem.56.7.2265-2267.1990

Plate Assay for Simultaneous Detection of Alginate Lyases and Determination of Substrate Specificity

Peter Gacesa 1,*, Frederick S Wusteman 1
PMCID: PMC184597  PMID: 16348243

Abstract

A plate assay to detect the presence of alginate lyases (EC 4.2.2.3) has been developed. The simultaneous use of specific alginate block structures of defined composition allows the substrate specificity of the enzymes to be determined. Clearing zones in the alginate-containing media are visualized with either cetyl pyridinium chloride or ruthenium red.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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