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. 1990 Jul;56(7):2268–2270. doi: 10.1128/aem.56.7.2268-2270.1990

Chromium reduction in Pseudomonas putida.

Y Ishibashi 1, C Cervantes 1, S Silver 1
PMCID: PMC184598  PMID: 2389940

Abstract

Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bopp L. H., Chakrabarty A. M., Ehrlich H. L. Chromate resistance plasmid in Pseudomonas fluorescens. J Bacteriol. 1983 Sep;155(3):1105–1109. doi: 10.1128/jb.155.3.1105-1109.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cervantes C., Ohtake H., Chu L., Misra T. K., Silver S. Cloning, nucleotide sequence, and expression of the chromate resistance determinant of Pseudomonas aeruginosa plasmid pUM505. J Bacteriol. 1990 Jan;172(1):287–291. doi: 10.1128/jb.172.1.287-291.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gvozdiak P. I., Mogilevich N. F., Ryl'skii A. F., Grishchenko N. I. Vosstanovlenie shestivalentnogo khroma kollektsionnymi shtammami bakterii. Mikrobiologiia. 1986 Nov-Dec;55(6):962–965. [PubMed] [Google Scholar]
  4. Izaki K., Tashiro Y., Funaba T. Mechanism of mercuric chloride resistance in microorganisms. 3. Purification and properties of a mercuric ion reducing enzyme from Escherichia coli bearing R factor. J Biochem. 1974 Mar;75(3):591–599. doi: 10.1093/oxfordjournals.jbchem.a130427. [DOI] [PubMed] [Google Scholar]
  5. Lebedeva E. V., Lialikova N. N. Vosstanovlenie krokoita kul'turoi Pseudomonas chromatophila sp. nov. Mikrobiologiia. 1979 May-Jun;48(3):517–522. [PubMed] [Google Scholar]
  6. Ohtake H., Cervantes C., Silver S. Decreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid. J Bacteriol. 1987 Aug;169(8):3853–3856. doi: 10.1128/jb.169.8.3853-3856.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wang P. C., Mori T., Komori K., Sasatsu M., Toda K., Ohtake H. Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions. Appl Environ Microbiol. 1989 Jul;55(7):1665–1669. doi: 10.1128/aem.55.7.1665-1669.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wang P. C., Mori T., Toda K., Ohtake H. Membrane-associated chromate reductase activity from Enterobacter cloacae. J Bacteriol. 1990 Mar;172(3):1670–1672. doi: 10.1128/jb.172.3.1670-1672.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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