Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Jul;56(7):2274–2275. doi: 10.1128/aem.56.7.2274-2275.1990

Replica plating of colonies from Listeria-selective agars to blood agar to improve the isolation of Listeria monocytogenes from foods.

P K Cassiday 1, L M Graves 1, B Swaminathan 1
PMCID: PMC184600  PMID: 2117884

Abstract

Bacterial colonies from Listeria-selective agars were replica plated to sheep blood agar to screen for beta-hemolysis. By using the replica plating method to test for the beta-hemolytic characteristic of all the colonies growing on Listeria-selective agars instead of picking 3 to 10 suspected colonies for further testing, we recovered Listeria monocytogenes from 59 of 142 Listeria-selective agar plates which contained colonies of hemolytic and nonhemolytic Listeria species and were negative when tested by conventional colony picks.

Full text

PDF
2274

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butman B. T., Plank M. C., Durham R. J., Mattingly J. A. Monoclonal antibodies which identify a genus-specific Listeria antigen. Appl Environ Microbiol. 1988 Jun;54(6):1564–1569. doi: 10.1128/aem.54.6.1564-1569.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Datta A. R., Wentz B. A., Hill W. E. Identification and enumeration of beta-hemolytic Listeria monocytogenes in naturally contaminated dairy products. J Assoc Off Anal Chem. 1988 May-Jun;71(3):673–675. [PubMed] [Google Scholar]
  3. Fleming D. W., Cochi S. L., MacDonald K. L., Brondum J., Hayes P. S., Plikaytis B. D., Holmes M. B., Audurier A., Broome C. V., Reingold A. L. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. doi: 10.1056/NEJM198502143120704. [DOI] [PubMed] [Google Scholar]
  4. Gray M. L., Stafseth H. J., Thorp F., Sholl L. B., Riley W. F. A New Technique for Isolating Listerellae from the Bovine Brain. J Bacteriol. 1948 Apr;55(4):471–476. [PMC free article] [PubMed] [Google Scholar]
  5. Klinger J. D., Johnson A., Croan D., Flynn P., Whippie K., Kimball M., Lawrie J., Curiale M. Comparative studies of nucleic acid hybridization assay for Listeria in foods. J Assoc Off Anal Chem. 1988 May-Jun;71(3):669–673. [PubMed] [Google Scholar]
  6. Lee W. H., McClain D. Improved Listeria monocytogenes selective agar. Appl Environ Microbiol. 1986 Nov;52(5):1215–1217. doi: 10.1128/aem.52.5.1215-1217.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
  8. van Netten P., Perales I., van de Moosdijk A., Curtis G. D., Mossel D. A. Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp. Int J Food Microbiol. 1989 Jul;8(4):299–316. doi: 10.1016/0168-1605(89)90001-9. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES