Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1983 Mar;23(3):503–505. doi: 10.1128/aac.23.3.503

Comparative activity of amantadine and ribavirin against influenza virus in vitro: possible clinical relevance.

M J Browne, M Y Moss, M R Boyd
PMCID: PMC184681  PMID: 6847176

Abstract

The activities of amantadine and ribavirin against influenza A viruses were compared against low-multiplicity (plaque inhibition) and high-multiplicity (protein synthesis inhibition) infections. Our results suggest that the predictive value of in vitro data for the clinic may be improved by consideration of tests against a high-multiplicity infection.

Full text

PDF
503

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard G. Amantadine-resistance as a genetic marker for influenza viruses. J Gen Virol. 1977 Aug;36(2):249–255. doi: 10.1099/0022-1317-36-2-249. [DOI] [PubMed] [Google Scholar]
  2. Appleyard G., Maber H. B. A plaque assay for the study of influenza virus inhibitors. J Antimicrob Chemother. 1975 Dec;1(4 Suppl):49–53. doi: 10.1093/jac/1.suppl_4.49. [DOI] [PubMed] [Google Scholar]
  3. Browne M. J. Comparative inhibition of influenza and parainfluenza virus replication by ribavirin in MDCK cells. Antimicrob Agents Chemother. 1981 May;19(5):712–715. doi: 10.1128/aac.19.5.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Browne M. J. Mechanism and specificity of action of ribavirin. Antimicrob Agents Chemother. 1979 Jun;15(6):747–753. doi: 10.1128/aac.15.6.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen A., Togo Y., Khakoo R., Waldman R., Sigel M. Comparative clinical and laboratory evaluation of the prophylactic capacity of ribavirin, amantadine hydrochloride, and placebo in induced human influenza type A. J Infect Dis. 1976 Jun;133 (Suppl):A114–A120. doi: 10.1093/infdis/133.supplement_2.a114. [DOI] [PubMed] [Google Scholar]
  6. Couch R. B., Jackson G. G. Antiviral agents in influenza--summary of Influenza Workshop VIII. J Infect Dis. 1976 Nov;134(5):516–527. doi: 10.1093/infdis/134.5.516. [DOI] [PubMed] [Google Scholar]
  7. Hayden F. G., Cote K. M., Douglas R. G., Jr Plaque inhibition assay for drug susceptibility testing of influenza viruses. Antimicrob Agents Chemother. 1980 May;17(5):865–870. doi: 10.1128/aac.17.5.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayden F. G., Hall W. J., Douglas R. G., Jr Therapeutic effects of aerosolized amantadine in naturally acquired infection due to influenza A virus. J Infect Dis. 1980 May;141(5):535–542. doi: 10.1093/infdis/141.5.535. [DOI] [PubMed] [Google Scholar]
  9. Knight V., Fedson D., Baldini J., Douglas R. G., Couch R. B. Amantadine therapy of epidemic influenza a(2) (Hong Kong). Infect Immun. 1970 Feb;1(2):200–204. doi: 10.1128/iai.1.2.200-204.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knight V., McClung H. W., Wilson S. Z., Waters B. K., Quarles J. M., Cameron R. W., Greggs S. E., Zerwas J. M., Couch R. B. Ribavirin small-particle aerosol treatment of influenza. Lancet. 1981 Oct 31;2(8253):945–949. doi: 10.1016/s0140-6736(81)91152-1. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lubeck M. D., Schulman J. L., Palese P. Susceptibility of influenza A viruses to amantadine is influenced by the gene coding for M protein. J Virol. 1978 Dec;28(3):710–716. doi: 10.1128/jvi.28.3.710-716.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Magnussen C. R., Douglas R. G., Jr, Betts R. F., Roth F. K., Meagher M. P. Double-blind evaluation of oral ribavirin (Virazole) in experimental influenza A virus infection in volunteers. Antimicrob Agents Chemother. 1977 Oct;12(4):498–502. doi: 10.1128/aac.12.4.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oxford J. S. Effects of 1-beta-D-ribofuranosyl 1, 2, 4-triazole-3-carboxamide on influenza virus replication and polypeptide synthesis. J Antimicrob Chemother. 1975 Dec;1(4 Suppl):71–76. doi: 10.1093/jac/1.suppl_4.71. [DOI] [PubMed] [Google Scholar]
  15. Oxford J. S. Inhibition of the replication of influenza A and B viruses by a nucleoside analogue (ribavirin). J Gen Virol. 1975 Sep;28(3):409–414. doi: 10.1099/0022-1317-28-3-409. [DOI] [PubMed] [Google Scholar]
  16. Oxford J. S. Specific inhibitors of influenza virus replication as potential chemoprophylactic agents. J Antimicrob Chemother. 1975 Mar;1(1):7–23. doi: 10.1093/jac/1.1.7. [DOI] [PubMed] [Google Scholar]
  17. Salido-Rengell F., Nasser-Quinones H., Briseno-Garcia B. Clinical evaluation of 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) in a double-blind study during an outbreak of influenza. Ann N Y Acad Sci. 1977 Mar 4;284:272–277. doi: 10.1111/j.1749-6632.1977.tb21960.x. [DOI] [PubMed] [Google Scholar]
  18. Scholtissek C., Faulkner G. P. Amantadine-resistant and -sensitive influenza A strains and recombinants. J Gen Virol. 1979 Sep;44(3):807–815. doi: 10.1099/0022-1317-44-3-807. [DOI] [PubMed] [Google Scholar]
  19. Skehel J. J., Hay A. J., Armstrong J. A. On the mechanism of inhibition of influenza virus replication by amantadine hydrochloride. J Gen Virol. 1978 Jan;38(1):97–110. doi: 10.1099/0022-1317-38-1-97. [DOI] [PubMed] [Google Scholar]
  20. Smith C. B., Charette R. P., Fox J. P., Cooney M. K., Hall C. E. Lack of effect of oral ribavirin in naturally occurring influenza A virus (H1N1) infection. J Infect Dis. 1980 May;141(5):548–554. doi: 10.1093/infdis/141.5.548. [DOI] [PubMed] [Google Scholar]
  21. Tisdale M., Bauer D. J. The relative potencies of anti-influenza compounds. Ann N Y Acad Sci. 1977 Mar 4;284:254–263. doi: 10.1111/j.1749-6632.1977.tb21958.x. [DOI] [PubMed] [Google Scholar]
  22. Tobita K., Sugiura A., Enomote C., Furuyama M. Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Med Microbiol Immunol. 1975 Dec 30;162(1):9–14. doi: 10.1007/BF02123572. [DOI] [PubMed] [Google Scholar]
  23. Togo Y. In vitro effect of virazole against influenza viruses. Antimicrob Agents Chemother. 1973 Dec;4(6):641–642. doi: 10.1128/aac.4.6.641. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES