Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1983 Apr;23(4):614–616. doi: 10.1128/aac.23.4.614

Importance of minimizing carry-over effect at subculture in the detection of penicillin-tolerant viridans group streptococci.

J Dankert, Y Holloway, W Joldersma, J Hess
PMCID: PMC184711  PMID: 6602588

Abstract

Penicillin susceptibility determinations made for 14 strains of Streptococcus mitior by two different broth dilution tests revealed small numbers of tolerant strains regardless of the volumes (0.01 and 0.1 ml) of subcultured broth. The addition of penicillinase to the subculture medium increased the number of tolerant strains significantly in 0.1 ml of subcultured broth, demonstrating that the detection of penicillin-tolerant viridans group streptococci might be hampered by the carry-over of penicillin.

Full text

PDF
614

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Lasner R. A. In-vitro methods for determining minimal lethal concentrations of antimicrobial agents. Am J Clin Pathol. 1979 Jan;71(1):88–92. doi: 10.1093/ajcp/71.1.88. [DOI] [PubMed] [Google Scholar]
  2. Bradley H. E., Weldy P. L., Hodes D. S. Tolerance in Staphylococcus aureus. Lancet. 1979 Jan 20;1(8108):150–150. doi: 10.1016/s0140-6736(79)90536-1. [DOI] [PubMed] [Google Scholar]
  3. Holloway Y., Dankert J., Hess J. Penicillin tolerance and bacterial endocarditis. Lancet. 1980 Mar 15;1(8168 Pt 1):589–589. doi: 10.1016/s0140-6736(80)91070-3. [DOI] [PubMed] [Google Scholar]
  4. Holloway Y., Schaareman M., Dankert J. Identification of viridans streptococci on the Minitek Miniaturised Differentiation System. J Clin Pathol. 1979 Nov;32(11):1168–1173. doi: 10.1136/jcp.32.11.1168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Horne D., Tomasz A. Lethal effect of a heterologous murein hydrolase on penicillin-treated Streptococcus sanguis. Antimicrob Agents Chemother. 1980 Feb;17(2):235–246. doi: 10.1128/aac.17.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kim K. S., Anthony B. F. Penicillin tolerance in group B streptococci isolated from infected neonates. J Infect Dis. 1981 Nov;144(5):411–419. doi: 10.1093/infdis/144.5.411. [DOI] [PubMed] [Google Scholar]
  7. Pearson R. D., Steigbigel R. T., Davis H. T., Chapman S. W. Method of reliable determination of minimal lethal antibiotic concentrations. Antimicrob Agents Chemother. 1980 Nov;18(5):699–708. doi: 10.1128/aac.18.5.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Portnoy D., Prentis J., Richards G. K. Penicillin tolerance of human isolates of group C streptococci. Antimicrob Agents Chemother. 1981 Aug;20(2):235–238. doi: 10.1128/aac.20.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Russell A. D., Ahonkhai I., Rogers D. T. Microbiological applications of the inactivation of antibiotics and other antimicrobial agents. J Appl Bacteriol. 1979 Apr;46(2):207–245. doi: 10.1111/j.1365-2672.1979.tb00818.x. [DOI] [PubMed] [Google Scholar]
  10. Sabath L. D., Wheeler N., Laverdiere M., Blazevic D., Wilkinson B. J. A new type of penicillin resistance of Staphylococcus aureus. Lancet. 1977 Feb 26;1(8009):443–447. doi: 10.1016/s0140-6736(77)91941-9. [DOI] [PubMed] [Google Scholar]
  11. Washington J. A., 2nd The role of the microbiology laboratory in the diagnosis and antimicrobial treatment of infective endocarditis. Mayo Clin Proc. 1982 Jan;57(1):22–32. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES