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. 1990 Aug;56(8):2296–2298. doi: 10.1128/aem.56.8.2296-2298.1990

Production of fumonisin B1 by Fusarium moniliforme NRRL 13616 in submerged culture.

M A Jackson 1, G A Bennett 1
PMCID: PMC184725  PMID: 2403248

Abstract

Fumonisin B1, a recently discovered mycotoxin, was synthesized by submerged cultures of Fusarium moniliforme NRRL 13616 grown for 29 days at 28 degrees C and 220 rpm in a basal salts medium (pH 5.0) supplemented with 90 g of glucose per liter and 3.5 g of ammonium sulfate per liter. Under these culture conditions, 74 +/- 23 micrograms of fumonisin B1 per ml was produced by 29-day-old F. moniliforme NRRL 13616 cultures. Fumonisin B1 was detected in liquid culture extracts by high-performance thin-layer chromatography. Fumonisin B1 was confirmed and quantitated by gas chromatography and gas chromatography-mass spectral analysis of the trimethylsilyl derivative. The use of a defined medium for producing fumonisin B1 in a submerged culture facilitates its isolation and provides an excellent method for conducting biosynthetic studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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