Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Aug;56(8):2436–2440. doi: 10.1128/aem.56.8.2436-2440.1990

Description of a Novel Symbiotic Bacterium from the Brittle Star, Amphipholis squamata

Michael P Lesser 1,†,*, Richard P Blakemore 1
PMCID: PMC184746  PMID: 16348257

Abstract

A gram-negative, marine, facultatively anaerobic bacterial isolate designated strain AS-1 was isolated from the subcuticular space of the brittle star, Amphipholis squamata. Its sensitivity to O/129 and novobiocin, overall morphology, and biochemical characteristics and the moles percent guanine-plus-cytosine composition of its DNA (42.9 to 44.4) suggest that this isolate should be placed in the genus Vibrio. Strain AS-1 was not isolated from ambient seawater and is distinct from described Vibrio species. This symbiotic bacterium may assist its host as one of several mechanisms of nutrient acquisition during the brooding of developing embryos.

Full text

PDF
2436

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker D. A., Park R. W. Changes in morphology and cell wall structure that occur during growth of Vibrio sp. NCTC4716 in batch culture. J Gen Microbiol. 1975 Jan;86(1):12–28. doi: 10.1099/00221287-86-1-12. [DOI] [PubMed] [Google Scholar]
  2. Cavanaugh C. M., Gardiner S. L., Jones M. L., Jannasch H. W., Waterbury J. B. Prokaryotic Cells in the Hydrothermal Vent Tube Worm Riftia pachyptila Jones: Possible Chemoautotrophic Symbionts. Science. 1981 Jul 17;213(4505):340–342. doi: 10.1126/science.213.4505.340. [DOI] [PubMed] [Google Scholar]
  3. Childress J. J., Fisher C. R., Brooks J. M., Kennicutt M. C., 2nd, Bidigare R., Anderson A. E. A methanotrophic marine molluscan (bivalvia, mytilidae) symbiosis: mussels fueled by gas. Science. 1986 Sep 19;233(4770):1306–1308. doi: 10.1126/science.233.4770.1306. [DOI] [PubMed] [Google Scholar]
  4. Costerton J. W., Ingram J. M., Cheng K. J. Structure and function of the cell envelope of gram-negative bacteria. Bacteriol Rev. 1974 Mar;38(1):87–110. doi: 10.1128/br.38.1.87-110.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Costerton J. W., Irvin R. T., Cheng K. J. The bacterial glycocalyx in nature and disease. Annu Rev Microbiol. 1981;35:299–324. doi: 10.1146/annurev.mi.35.100181.001503. [DOI] [PubMed] [Google Scholar]
  6. Felbeck H. Chemoautotrophic Potential of the Hydrothermal Vent Tube Worm, Riftia pachyptila Jones (Vestimentifera). Science. 1981 Jul 17;213(4505):336–338. doi: 10.1126/science.213.4505.336. [DOI] [PubMed] [Google Scholar]
  7. Fontaine A. R., Chia F. S. Echinoderms: an autoradiographic study of assimilation of dissolved organic molecules. Science. 1968 Sep 13;161(3846):1153–1155. doi: 10.1126/science.161.3846.1153. [DOI] [PubMed] [Google Scholar]
  8. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  9. MacDonell M. T., Singleton F. L., Hood M. A. Diluent composition for use of API 20E in characterizing marine and estuarine bacteria. Appl Environ Microbiol. 1982 Aug;44(2):423–427. doi: 10.1128/aem.44.2.423-427.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ochi A., Hawley R. G., Shulman M. J., Hozumi N. Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody production. Nature. 1983 Mar 24;302(5906):340–342. doi: 10.1038/302340a0. [DOI] [PubMed] [Google Scholar]
  11. Waterbury J. B., Calloway C. B., Turner R. D. A cellulolytic nitrogen-fixing bacterium cultured from the gland of deshayes in shipworms (bivalvia: teredinidae). Science. 1983 Sep 30;221(4618):1401–1403. doi: 10.1126/science.221.4618.1401. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES