Abstract
Microorganisms utilizing an opine as the sole carbon source were recovered from crown gall tumors, soil, and surface-disinfected potato tubers. The effect of the opines octopine, nopaline, succinamopine, and mannopine as selective substrates was compared with that of the auxin indoleacetic acid. Selection on octopine and indoleacetic acid favored the fluorescent pseudomonads, whereas mannopine allowed the frequent recovery of agrobacteria. Coryneforms which utilized succinamopine or mannopine were detected in soil, but not in tumors. Fungi growing on succinamopine or mannopine and a mannopine-utilizing Pseudomonas putida were isolated from tumor and soil, respectively.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beauchamp C. J., Chilton W. S., Dion P., Antoun H. Fungal catabolism of crown gall opines. Appl Environ Microbiol. 1990 Jan;56(1):150–155. doi: 10.1128/aem.56.1.150-155.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bouzar H., Moore L. W. Isolation of different agrobacterium biovars from a natural oak savanna and tallgrass prairie. Appl Environ Microbiol. 1987 Apr;53(4):717–721. doi: 10.1128/aem.53.4.717-721.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cangelosi G. A., Hung L., Puvanesarajah V., Stacey G., Ozga D. A., Leigh J. A., Nester E. W. Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. J Bacteriol. 1987 May;169(5):2086–2091. doi: 10.1128/jb.169.5.2086-2091.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dessaux Y., Tempé J., Farrand S. K. Genetic analysis of mannityl opine catabolism in octopine-type Agrobacterium tumefaciens strain 15955. Mol Gen Genet. 1987 Jun;208(1-2):301–308. doi: 10.1007/BF00330457. [DOI] [PubMed] [Google Scholar]
- Dylan T., Ielpi L., Stanfield S., Kashyap L., Douglas C., Yanofsky M., Nester E., Helinski D. R., Ditta G. Rhizobium meliloti genes required for nodule development are related to chromosomal virulence genes in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4403–4407. doi: 10.1073/pnas.83.12.4403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gordon S. A., Weber R. P. COLORIMETRIC ESTIMATION OF INDOLEACETIC ACID. Plant Physiol. 1951 Jan;26(1):192–195. doi: 10.1104/pp.26.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guyon P., Chilton M. D., Petit A., Tempé J. Agropine in "null-type" crown gall tumors: Evidence for generality of the opine concept. Proc Natl Acad Sci U S A. 1980 May;77(5):2693–2697. doi: 10.1073/pnas.77.5.2693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayman G. T., Farrand S. K. Characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline Ti plasmid pTiC58. J Bacteriol. 1988 Apr;170(4):1759–1767. doi: 10.1128/jb.170.4.1759-1767.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holsters M., Silva B., Van Vliet F., Genetello C., De Block M., Dhaese P., Depicker A., Inzé D., Engler G., Villarroel R. The functional organization of the nopaline A. tumefaciens plasmid pTiC58. Plasmid. 1980 Mar;3(2):212–230. doi: 10.1016/0147-619x(80)90110-9. [DOI] [PubMed] [Google Scholar]
- Stachel S. E., Nester E. W. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens. EMBO J. 1986 Jul;5(7):1445–1454. doi: 10.1002/j.1460-2075.1986.tb04381.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tremblay G., Gagliardo R., Chilton W. S., Dion P. Diversity among Opine-Utilizing Bacteria: Identification of Coryneform Isolates. Appl Environ Microbiol. 1987 Jul;53(7):1519–1524. doi: 10.1128/aem.53.7.1519-1524.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unger L., Ziegler S. F., Huffman G. A., Knauf V. C., Peet R., Moore L. W., Gordon M. P., Nester E. W. New class of limited-host-range Agrobacterium mega-tumor-inducing plasmids lacking homology to the transferred DNA of a wide-host-range, tumor-inducing plasmid. J Bacteriol. 1985 Nov;164(2):723–730. doi: 10.1128/jb.164.2.723-730.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
