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. 1990 Sep;56(9):2736–2741. doi: 10.1128/aem.56.9.2736-2741.1990

Hemoproteins of Bradyrhizobium japonicum Cultured Cells and Bacteroids

Donald L Keister 1,*, Sarah S Marsh 1
PMCID: PMC184835  PMID: 16348282

Abstract

The hemoprotein content of 17 strains of Bradyrhizobium japonicum bacteroids from field-grown plants and the corresponding strains of cultured cells was determined spectrally. The major terminal oxidases, cytochromes (cyt) aa3 and o, were present in all strains of cultured cells. cyt aa3 was present in significant amounts in bacteroids only in strains of DNA homology group II. cyt o appeared to be present in bacteroids of all strains, and the average level was the same as in cultured cells. cyt b and c in the membrane fractions were higher in bacteroids of all strains compared with cultured cells. cyt P-450 was present in both the membrane and soluble fractions of bacteroids of most strains. The total P-450 content varied sixfold among strains. A CO-reactive hemoprotein, P-422, was present in the soluble fraction of all strains of cultured cells. P-422 may be a hemoglobinlike protein, and it was present in significant amounts in bacteroids only in DNA homology group I strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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