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. 1990 Sep;56(9):2761–2763. doi: 10.1128/aem.56.9.2761-2763.1990

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O2†

Ching-Ping Tseng 2, Thomas J Montville 2,*
PMCID: PMC184839  PMID: 16348283

Abstract

Lactobacillus plantarum catabolic end products changed in response to environmental conditions. While lactate was always the major end product, acetate was produced in alkaline and aerobic environments. Acetoin levels decreased under alkaline conditions. Changes in acetoin dehydrogenase, acetate kinase, NADH oxidase, pyruvate oxidase, and acetate kinase activities correlated with changes in end product distribution.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Bresters T. W., Krul J., Scheepens P. C., Veeger C. Phosphotransacetylase associated with the pyruvate dehydrogenase complex from the nitrogen fixing Azotobacter vinelandii. FEBS Lett. 1972 May 15;22(3):305–309. doi: 10.1016/0014-5793(72)80257-6. [DOI] [PubMed] [Google Scholar]
  3. CRAIG J. A., SNELL E. E. The comparative activities of pantethine, pantothenic acid, and coenzyme A for various microorganisms. J Bacteriol. 1951 Mar;61(3):283–291. doi: 10.1128/jb.61.3.283-291.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlsson J., Kujala U., Edlund M. B. Pyruvate dehydrogenase activity in Streptococcus mutans. Infect Immun. 1985 Sep;49(3):674–678. doi: 10.1128/iai.49.3.674-678.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DENNIS D., KAPLAN N. O. D- and L-lactic acid dehydrogenases in Lactobacillus plantarum. J Biol Chem. 1960 Mar;235:810–818. [PubMed] [Google Scholar]
  6. Garvie E. I. Bacterial lactate dehydrogenases. Microbiol Rev. 1980 Mar;44(1):106–139. doi: 10.1128/mr.44.1.106-139.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Götz F., Schleifer K. H. Biochemical properties and the physiological role of the fructose-1,6-bisphosphate activated L-lactate dehydrogenase from Staphylococcus epidermidis. Eur J Biochem. 1978 Oct 16;90(3):555–561. doi: 10.1111/j.1432-1033.1978.tb12635.x. [DOI] [PubMed] [Google Scholar]
  8. Götz F., Sedewitz B., Elstner E. F. Oxygen utilization by Lactobacillus plantarum. I. Oxygen consuming reactions. Arch Microbiol. 1980 Apr;125(3):209–214. doi: 10.1007/BF00446878. [DOI] [PubMed] [Google Scholar]
  9. Hickey M. W., Hillier A. J., Jago G. R. Metabolism of pyruvate and citrate in lactobacilli. Aust J Biol Sci. 1983;36(5-6):487–496. doi: 10.1071/bi9830487. [DOI] [PubMed] [Google Scholar]
  10. López J., Fortinagel P. The regulation of the butanediol cycle in Bacillus subtilis. Biochim Biophys Acta. 1972 Oct 25;279(3):554–560. doi: 10.1016/0304-4165(72)90177-8. [DOI] [PubMed] [Google Scholar]
  11. MIZUSHIMA S., KITAHARA K. QUANTITATIVE STUDIES ON GLYCOLYTIC ENZYMES IN LACTOBACILLUS PLANTARUM. II. INTRACELLULAR CONCENTRATIONS OF GLYCOLYTIC INTERMEDIATES IN GLUCOSE-METABOLIZING WASHED CELLS. J Bacteriol. 1964 Jun;87:1429–1435. doi: 10.1128/jb.87.6.1429-1435.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Melville S. B., Michel T. A., Macy J. M. Pathway and sites for energy conservation in the metabolism of glucose by Selenomonas ruminantium. J Bacteriol. 1988 Nov;170(11):5298–5304. doi: 10.1128/jb.170.11.5298-5304.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Montville T. J., Hsu A. H., Meyer M. E. High-Efficiency Conversion of Pyruvate to Acetoin by Lactobacillus plantarum during pH-Controlled and Fed-Batch Fermentations. Appl Environ Microbiol. 1987 Aug;53(8):1798–1802. doi: 10.1128/aem.53.8.1798-1802.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy M. G., Condon S. Correlation of oxygen utilization and hydrogen peroxide accumulation with oxygen induced enzymes in Lactobacillus plantarum cultures. Arch Microbiol. 1984 May;138(1):44–48. doi: 10.1007/BF00425405. [DOI] [PubMed] [Google Scholar]
  15. Murphy M. G., O'Connor L., Walsh D., Condon S. Oxygen dependent lactate utilization by Lactobacillus plantarum. Arch Microbiol. 1985 Feb;141(1):75–79. doi: 10.1007/BF00446743. [DOI] [PubMed] [Google Scholar]
  16. Nakajima H., Suzuki K., Imahori K. Purification and properties of acetate kinase from Bacillus stearothermophilus. J Biochem. 1978 Jul;84(1):193–203. doi: 10.1093/oxfordjournals.jbchem.a132108. [DOI] [PubMed] [Google Scholar]
  17. Rhee S. K., Pack M. Y. Effect of environmental pH on fermentation balance of Lactobacillus bulgaricus. J Bacteriol. 1980 Oct;144(1):217–221. doi: 10.1128/jb.144.1.217-221.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sedewitz B., Schleifer K. H., Götz F. Purification and biochemical characterization of pyruvate oxidase from Lactobacillus plantarum. J Bacteriol. 1984 Oct;160(1):273–278. doi: 10.1128/jb.160.1.273-278.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takahashi S., Abbe K., Yamada T. Purification of pyruvate formate-lyase from Streptococcus mutans and its regulatory properties. J Bacteriol. 1982 Mar;149(3):1034–1040. doi: 10.1128/jb.149.3.1034-1040.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thomas T. D., Ellwood D. C., Longyear V. M. Change from homo- to heterolactic fermentation by Streptococcus lactis resulting from glucose limitation in anaerobic chemostat cultures. J Bacteriol. 1979 Apr;138(1):109–117. doi: 10.1128/jb.138.1.109-117.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. de Vries W., Kapteijn W. M., van der Beek E. G., Stouthamer A. H. Molar growth yields and fermentation balances of Lactobacillus casei L3 in batch cultures and in continuous cultures. J Gen Microbiol. 1970 Nov;63(3):333–345. doi: 10.1099/00221287-63-3-333. [DOI] [PubMed] [Google Scholar]
  22. ten Brink B., Konings W. N. Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol. 1982 Nov;152(2):682–686. doi: 10.1128/jb.152.2.682-686.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. ten Brink B., Otto R., Hansen U. P., Konings W. N. Energy recycling by lactate efflux in growing and nongrowing cells of Streptococcus cremoris. J Bacteriol. 1985 Apr;162(1):383–390. doi: 10.1128/jb.162.1.383-390.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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