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. 1990 Sep;56(9):2933–2935. doi: 10.1128/aem.56.9.2933-2935.1990

Preservation of Ruminal Bacterium Capsules by Using Lysine in the Electron Microscopy Fixative

D E Akin 1,*, L L Rigsby 1
PMCID: PMC184870  PMID: 2125818

Abstract

Ruminal bacteria from axenic cultures of Ruminococcus flavefaciens FD1, Butyrivibrio fibrisolvens 49, and bacterial types from the ruminal ecosystem that were fixed with 50 mM lysine (l-lysine hydrochloride) added to glutaraldehyde had better-preserved capsules and extracellular material than bacteria fixed without lysine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akin D. E., Burdick D., Michaels G. E. Rumen bacterial interrelationships with plant tissue during degradation revealed by transmission electron microscopy. Appl Microbiol. 1974 Jun;27(6):1149–1156. doi: 10.1128/am.27.6.1149-1156.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Akin D. E. Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls. Appl Environ Microbiol. 1980 Jan;39(1):242–252. doi: 10.1128/aem.39.1.242-252.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Akin D. E. Ultrastructure of rumen bacterial attachment to forage cell walls. Appl Environ Microbiol. 1976 Apr;31(4):562–568. doi: 10.1128/aem.31.4.562-568.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boyles J., Anderson L., Hutcherson P. A new fixative for the preservation of actin filaments: fixation of pure actin filament pellets. J Histochem Cytochem. 1985 Nov;33(11):1116–1128. doi: 10.1177/33.11.3902963. [DOI] [PubMed] [Google Scholar]
  5. Cheng K. J., Costerton J. W. Ultrastructure of Butyrivibrio fibrisolvens: a gram-positive bacterium. J Bacteriol. 1977 Mar;129(3):1506–1512. doi: 10.1128/jb.129.3.1506-1512.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cheng K. J., Costerton J. W. Ultrastructure of cell envelopes of bacteria of the bovine rumen. Appl Microbiol. 1975 Jun;29(6):841–849. doi: 10.1128/am.29.6.841-849.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jacques M., Graham L. Improved preservation of bacterial capsule for electron microscopy. J Electron Microsc Tech. 1989 Feb;11(2):167–169. doi: 10.1002/jemt.1060110212. [DOI] [PubMed] [Google Scholar]
  8. Latham M. J., Brooker B. E., Pettipher G. L., Harris P. J. Adhesion of Bacteroides succinogenes in pure culture and in the presence of Ruminococcus flavefaciens to cell walls in leaves of perennial ryegrass (Lolium perenne). Appl Environ Microbiol. 1978 Jun;35(6):1166–1173. doi: 10.1128/aem.35.6.1166-1173.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spurr A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969 Jan;26(1):31–43. doi: 10.1016/s0022-5320(69)90033-1. [DOI] [PubMed] [Google Scholar]
  10. Stack R. J., Hungate R. E. Effect of 3-Phenylpropanoic Acid on Capsule and Cellulases of Ruminococcus albus 8. Appl Environ Microbiol. 1984 Jul;48(1):218–223. doi: 10.1128/aem.48.1.218-223.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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