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. 1990 Oct;56(10):3164–3169. doi: 10.1128/aem.56.10.3164-3169.1990

Purification and Characterization of an Aspecific Glycoside Hydrolase from the Anaerobic Ruminal Fungus Neocallimastix frontalis

M Hebraud 1,†,*, M Fevre 1
PMCID: PMC184916  PMID: 16348324

Abstract

A glycoside hydrolase characterized by β-fucosidase (EC 3.2.1.38) and β-glucosidase (EC 3.2.1.21) activities was purified from the culture medium of the anaerobic ruminal phycomycete Neocallimastix frontalis grown on 0.5% Avicel. The enzyme had a molecular mass of 120 kilodaltons and a pI of 3.85. Optimal activity against p-nitrophenyl-β-d-fucoside and p-nitrophenyl-β-D-glucoside occurred at pH 6.0 and 50°C. The β-fucosidase and β-glucosidase activities were stable from pH 6.0 to pH 7.8 and up to 40°C. They were both inhibited by gluconolactone, sodium dodecyl sulfate, p-chloromercuribenzoate, and Hg2+ cation. The enzyme had Kms of 0.26 mg/ml for p-nitrophenyl-β-d-fucoside and 0.08 mg/ml for p-nitrophenyl-β-d-glucoside. The purified protein also had low β-galactosidase activity.

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Selected References

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