Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Oct;56(10):3216–3219. doi: 10.1128/aem.56.10.3216-3219.1990

Thermotolerance of Listeria monocytogenes and Salmonella typhimurium after sublethal heat shock.

V K Bunning 1, R G Crawford 1, J T Tierney 1, J T Peeler 1
PMCID: PMC184925  PMID: 2126703

Abstract

The effect of prior heat shock on thermotolerance of Listeria monocytogenes and Salmonella typhimurium in broth culture was determined. Bacteria were grown at the permissive temperature of 35 degrees C, sublethally heated at 35 (control), 42, 48, and 52 degrees C (nonpermissive control) for various times, and inactivated at either 57.8 or 52 degrees C. The induction of increased thermotolerance by heat shock, although consistent within each experiment, was generally not significant for L. monocytogenes; the increase was significant for S. typhimurium. Temperature shift experiments with L. monocytogenes suggested that induced thermotolerance was not long lived unless the shock temperature was maintained.

Full text

PDF
3216

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bunning V. K., Crawford R. G., Bradshaw J. G., Peeler J. T., Tierney J. T., Twedt R. M. Thermal resistance of intracellular Listeria monocytogenes cells suspended in raw bovine milk. Appl Environ Microbiol. 1986 Dec;52(6):1398–1402. doi: 10.1128/aem.52.6.1398-1402.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bunning V. K., Donnelly C. W., Peeler J. T., Briggs E. H., Bradshaw J. G., Crawford R. G., Beliveau C. M., Tierney J. T. Thermal inactivation of Listeria monocytogenes within bovine milk phagocytes. Appl Environ Microbiol. 1988 Feb;54(2):364–370. doi: 10.1128/aem.54.2.364-370.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crawford R. G., Beliveau C. M., Peeler J. T., Donnelly C. W., Bunning V. K. Comparative recovery of uninjured and heat-injured Listeria monocytogenes cells from bovine milk. Appl Environ Microbiol. 1989 Jun;55(6):1490–1494. doi: 10.1128/aem.55.6.1490-1494.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doyle M. P., Glass K. A., Beery J. T., Garcia G. A., Pollard D. J., Schultz R. D. Survival of Listeria monocytogenes in milk during high-temperature, short-time pasteurization. Appl Environ Microbiol. 1987 Jul;53(7):1433–1438. doi: 10.1128/aem.53.7.1433-1438.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farber J. M., Brown B. E. Effect of prior heat shock on heat resistance of Listeria monocytogenes in meat. Appl Environ Microbiol. 1990 Jun;56(6):1584–1587. doi: 10.1128/aem.56.6.1584-1587.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farber J. M., Sanders G. W., Speirs J. I., D'Aoust J. Y., Emmons D. B., McKellar R. Thermal resistance of Listeria monocytogenes in inoculated and naturally contaminated raw milk. Int J Food Microbiol. 1988 Dec 31;7(4):277–286. doi: 10.1016/0168-1605(88)90054-2. [DOI] [PubMed] [Google Scholar]
  7. Knabel S. J., Walker H. W., Hartman P. A., Mendonca A. F. Effects of growth temperature and strictly anaerobic recovery on the survival of Listeria monocytogenes during pasteurization. Appl Environ Microbiol. 1990 Feb;56(2):370–376. doi: 10.1128/aem.56.2.370-376.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lund B. M., Knox M. R., Cole M. B. Destruction of Listeria monocytogenes during microwave cooking. Lancet. 1989 Jan 28;1(8631):218–218. doi: 10.1016/s0140-6736(89)91230-0. [DOI] [PubMed] [Google Scholar]
  9. Mackey B. M., Derrick C. M. Elevation of the heat resistance of Salmonella typhimurium by sublethal heat shock. J Appl Bacteriol. 1986 Nov;61(5):389–393. doi: 10.1111/j.1365-2672.1986.tb04301.x. [DOI] [PubMed] [Google Scholar]
  10. Ryan C. A., Nickels M. K., Hargrett-Bean N. T., Potter M. E., Endo T., Mayer L., Langkop C. W., Gibson C., McDonald R. C., Kenney R. T. Massive outbreak of antimicrobial-resistant salmonellosis traced to pasteurized milk. JAMA. 1987 Dec 11;258(22):3269–3274. [PubMed] [Google Scholar]
  11. Sanchez Y., Lindquist S. L. HSP104 required for induced thermotolerance. Science. 1990 Jun 1;248(4959):1112–1115. doi: 10.1126/science.2188365. [DOI] [PubMed] [Google Scholar]
  12. Sokolovic Z., Goebel W. Synthesis of listeriolysin in Listeria monocytogenes under heat shock conditions. Infect Immun. 1989 Jan;57(1):295–298. doi: 10.1128/iai.57.1.295-298.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sörqvist S. Heat resistance of Campylobacter and Yersinia strains by three methods. J Appl Bacteriol. 1989 Nov;67(5):543–549. doi: 10.1111/j.1365-2672.1989.tb02526.x. [DOI] [PubMed] [Google Scholar]
  14. VanBogelen R. A., Acton M. A., Neidhardt F. C. Induction of the heat shock regulon does not produce thermotolerance in Escherichia coli. Genes Dev. 1987 Aug;1(6):525–531. doi: 10.1101/gad.1.6.525. [DOI] [PubMed] [Google Scholar]
  15. Wilkins P. O., Bourgeois R., Murray R. G. Psychrotrophic properties of Listeria monocytogenes. Can J Microbiol. 1972 May;18(5):543–551. doi: 10.1139/m72-087. [DOI] [PubMed] [Google Scholar]
  16. Yamamori T., Yura T. Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982 Feb;79(3):860–864. doi: 10.1073/pnas.79.3.860. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES