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. 1990 Nov;56(11):3235–3239. doi: 10.1128/aem.56.11.3235-3239.1990

Effects of Partial O2 Pressure, Partial CO2 Pressure, and Agitation on Growth Kinetics of Azospirillum lipoferum under Fermentor Conditions

Etienne Paul 1, Daniel Mulard 1,†, Philippe Blanc 1, Jacques Fages 1,†, Gerard Goma 1, Alain Pareilleux 1,*
PMCID: PMC184935  PMID: 16348331

Abstract

Azospirillum lipoferum crt1 was grown in batch cultures under standard conditions at 85% saturation of dissolved oxygen (DO) and 30-g/liter glucose concentrations. Kinetic studies revealed nutritional limitations of growth and the presence of an initial lag phase prior to consumption of glucose. The influences of various gaseous environments and shear stress on growth, i.e., various conditions of agitation-aeration, were characterized. Faster growth in the first stages of the culture and shorter duration of the lag phase were observed at DO concentrations of <30% saturation. The possible influences of dissolved CO2 concentration or shear stress or both were discounted, and we confirmed the detrimental effect of high DO levels (up to 80% saturation) and the favorable influence of low DO concentrations (lower than 30% saturation) on growth. It was concluded that the gaseous environment, i.e., the DO concentration, needs to be considered as an operating parameter and be accurately controlled to ensure optimal growth of Azospirillum cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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