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. 1990 Nov;56(11):3298–3303. doi: 10.1128/aem.56.11.3298-3303.1990

Single-Strain versus Multistrain Inoculation: Effect of Soil Mineral N Availability on Rhizobial Strain Effectiveness and Competition for Nodulation on Chick-Pea, Soybean, and Dry Bean †

Padma Somasegaran 1,*, B Ben Bohlool 1
PMCID: PMC184945  PMID: 16348337

Abstract

The nitrogen-fixing effectiveness of multistrain inoculants was found to be determined by both the effectiveness of the component strains and the percentage of the nodules occupied by them. Multistrain formulations were always either as good as the most effective single-strain inoculant or intermediate between the most and the least effective. The percentage of nodules occupied and the amount of nitrogen fixed by the component strains of a multistrain inoculant showed highly significant linear correlation. The availability of soil N had a significant influence on the nitrogen fixation potential of each strain. The mineral N status of the soil was clearly a significant factor in affecting the competition pattern of Rhizobium loti (chick-pea) and Bradyrhizobium japonicum strains. Differences between the effectiveness of strains were masked under conditions of soil N availability. However, when soil N was immobilized with sugarcane bagasse, the differences became significant. In the chick-pea system, R. loti TAL 1148 (Nit 27A8) was the most effective but not the most competitive of the three strains used. In the soybean and dry bean systems, B. japonicum TAL 102 (USDA 110) and R. leguminosarum bv. phaseoli TAL 182, respectively, were consistently the most effective and, more often than not, the most competitive of the strains used for each species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohlool B. B., Schmidt E. L. Nonspecific staining: its control in immunofluorescence examination of soil. Science. 1968 Nov 29;162(3857):1012–1014. doi: 10.1126/science.162.3857.1012. [DOI] [PubMed] [Google Scholar]
  2. George T., Bohlool B. B., Singleton P. W. Bradyrhizobium japonicum-Environment Interactions: Nodulation and Interstrain Competition in Soils along an Elevational Transect. Appl Environ Microbiol. 1987 May;53(5):1113–1117. doi: 10.1128/aem.53.5.1113-1117.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kosslak R. M., Bohlool B. B. Influence of Environmental Factors on Interstrain Competition in Rhizobium japonicum. Appl Environ Microbiol. 1985 May;49(5):1128–1133. doi: 10.1128/aem.49.5.1128-1133.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McNeil D. L. Variations in Ability of Rhizobium japonicum Strains To Nodulate Soybeans and Maintain Fixation in the Presence of Nitrate. Appl Environ Microbiol. 1982 Sep;44(3):647–652. doi: 10.1128/aem.44.3.647-652.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Singleton P. W., Tavares J. W. Inoculation response of legumes in relation to the number and effectiveness of indigenous Rhizobium populations. Appl Environ Microbiol. 1986 May;51(5):1013–1018. doi: 10.1128/aem.51.5.1013-1018.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Somasegaran P., Martin R. B. Symbiotic Characteristics and Rhizobium Requirements of a Leucaena leucocephala x Leucaena diversifolia Hybrid and Its Parental Genotypes. Appl Environ Microbiol. 1986 Dec;52(6):1422–1424. doi: 10.1128/aem.52.6.1422-1424.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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