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. 1990 Nov;56(11):3412–3419. doi: 10.1128/aem.56.11.3412-3419.1990

Evidence for a Plasmid-Linked Restriction-Modification System in Lactobacillus helveticus

Clara G de los Reyes-Gavilán 1,†, Gaëtan K Y Limsowtin 1,‡, Laurent Séchaud 1, Monique Veaux 1, Jean-Pierre Accolas 1,*
PMCID: PMC184964  PMID: 16348347

Abstract

The presence of a restriction-modification (R/M) system against two bacteriophages, 328-B1 and hv, was demonstrated in three Lactobacillus helveticus strains, CNRZ 1094, CNRZ 1095, and CNRZ 1096. In addition, the burst size of phage 328-B1 in the three restrictive strains CNRZ 1094, CNRZ 1095, and CNRZ 1096 was reduced with respect to the values obtained in its propagating strain, CNRZ 328. Heating at 60°C did not inactivate the R/M system. Nonrestrictive variants from CNRZ 1094 were easily obtained under several culture conditions, but treatment with novobiocin at 42°C followed by storage at −20°C resulted in drastic elimination of the R+/M+ phenotype from all clones tested. Electrophoretic analysis of CNRZ 1094 nonrestrictive variants revealed the concomitant loss of a 34-kb plasmid. Four EcoRI fragments from the 34-kb plasmid were cloned in the Escherichia coli vector pACYC184. The use of one or several of these fragments as probes confirmed the plasmidic location of the genes responsible for the R/M system. These probes also showed the presence of R/M plasmids in the two other restrictive strains, CNRZ 1095 and CNRZ 1096. Lactose-fermenting ability and/or proteolytic capacity was not linked to the 34-kb plasmid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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