Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1990 Nov;56(11):3478–3481. doi: 10.1128/aem.56.11.3478-3481.1990

Detection of Listeria monocytogenes in foods by immunomagnetic separation.

E Skjerve 1, L M Rørvik 1, O Olsvik 1
PMCID: PMC184991  PMID: 2125186

Abstract

Immunomagnetic separation with immunomagnetic beads was used to isolate strains of Listeria monocytogenes both from pure cultures and from heterogeneous suspensions. The monoclonal antibodies used recognized all six strains of serotype 4 but only one of three strains of serotype 1. Coating procedure, incubation time, and number of immunomagnetic beads influenced the sensitivity of the isolation method. Less than 1 x 10(2) bacteria per ml in pure cultures and less than 2 x 10(2) bacteria per ml in enriched foods could be detected. The method represents a new approach to extraction and isolation of pathogenic bacteria directly from foods, after resuscitation, or from enrichment broths.

Full text

PDF
3478

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer D. L., Young F. E. Contemporary issues: diseases with a food vector. Clin Microbiol Rev. 1988 Oct;1(4):377–398. doi: 10.1128/cmr.1.4.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brackett R. E., Beuchat L. R. Methods and media for the isolation and cultivation of Listeria monocytogenes from various foods. Int J Food Microbiol. 1989 Jun;8(3):219–223. doi: 10.1016/0168-1605(89)90016-0. [DOI] [PubMed] [Google Scholar]
  3. Buchanan R. L., Stahl H. G., Bencivengo M. M., Del Corral F. Comparison of lithium chloride-phenylethanol-moxalactam and modified Vogel Johnson agars for detection of Listeria spp. in retail-level meats, poultry, and seafood. Appl Environ Microbiol. 1989 Mar;55(3):599–603. doi: 10.1128/aem.55.3.599-603.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butman B. T., Plank M. C., Durham R. J., Mattingly J. A. Monoclonal antibodies which identify a genus-specific Listeria antigen. Appl Environ Microbiol. 1988 Jun;54(6):1564–1569. doi: 10.1128/aem.54.6.1564-1569.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donnelly C. W., Baigent G. J., Briggs E. H. Flow cytometry for automated analysis of milk containing Listeria monocytogenes. J Assoc Off Anal Chem. 1988 May-Jun;71(3):655–658. [PubMed] [Google Scholar]
  6. Hill W. E., Ferreira J. L., Payne W. L., Jones V. M. Probability of recovering pathogenic Escherichia coli from foods. Appl Environ Microbiol. 1985 Jun;49(6):1374–1378. doi: 10.1128/aem.49.6.1374-1378.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johansen L., Nustad K., Orstavik T. B., Ugelstad J., Berge A., Ellingsen T. Excess antibody immunoassay for rat glandular kallikrein. Monosized polymer particles as the preferred solid phase material. J Immunol Methods. 1983 Apr 29;59(2):255–264. doi: 10.1016/0022-1759(83)90038-8. [DOI] [PubMed] [Google Scholar]
  8. Johne B., Jarp J. A rapid assay for protein-A in Staph. aureus strains, using immunomagnetic monosized polymer particles. APMIS. 1988 Jan;96(1):43–49. [PubMed] [Google Scholar]
  9. Johne B., Jarp J., Haaheim L. R. Staphylococcus aureus exopolysaccharide in vivo demonstrated by immunomagnetic separation and electron microscopy. J Clin Microbiol. 1989 Jul;27(7):1631–1635. doi: 10.1128/jcm.27.7.1631-1635.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. King W., Raposa S., Warshaw J., Johnson A., Halbert D., Klinger J. D. A new colorimetric nucleic acid hybridization assay for Listeria in foods. Int J Food Microbiol. 1989 Jun;8(3):225–232. doi: 10.1016/0168-1605(89)90017-2. [DOI] [PubMed] [Google Scholar]
  11. Klinger J. D., Johnson A., Croan D., Flynn P., Whippie K., Kimball M., Lawrie J., Curiale M. Comparative studies of nucleic acid hybridization assay for Listeria in foods. J Assoc Off Anal Chem. 1988 May-Jun;71(3):669–673. [PubMed] [Google Scholar]
  12. Lund A., Hellemann A. L., Vartdal F. Rapid isolation of K88+ Escherichia coli by using immunomagnetic particles. J Clin Microbiol. 1988 Dec;26(12):2572–2575. doi: 10.1128/jcm.26.12.2572-2575.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mattingly J. A., Butman B. T., Plank M. C., Durham R. J., Robison B. J. Rapid monoclonal antibody-based enzyme-linked immunosorbent assay for detection of Listeria in food products. J Assoc Off Anal Chem. 1988 May-Jun;71(3):679–681. [PubMed] [Google Scholar]
  14. McConway M. G., Chapman R. S. Application of solid-phase antibodies to radioimmunoassay. Evaluation of two polymeric microparticles, Dynospheres and nylon, activated by carbonyldiimidazole or tresyl chloride. J Immunol Methods. 1986 Dec 24;95(2):259–266. doi: 10.1016/0022-1759(86)90413-8. [DOI] [PubMed] [Google Scholar]
  15. Mossel D. A. Listeria monocytogenes in foods. Isolation, characterization and control. Int J Food Microbiol. 1989 Jun;8(3):183–195. doi: 10.1016/0168-1605(89)90013-5. [DOI] [PubMed] [Google Scholar]
  16. Nustad K., Closs O., Ugelstad J. Mouse monoclonal anti rabbit IgG coupled to monodisperse polymer particles. Comparison with polyclonal antibodies in radioimmunoassay for thyroid hormones. Dev Biol Stand. 1984;57:321–324. [PubMed] [Google Scholar]
  17. Nustad K., Monrad-Hansen H. P., Paus E., Millán J. L., Nørgaard-Pedersen B. Evaluation of a new, sensitive radioimmunoassay for placental alkaline phosphatase in pre- and post-operative sera from the Danish testicular cancer material. Prog Clin Biol Res. 1984;166:337–348. [PubMed] [Google Scholar]
  18. Peel M., Donachie W., Shaw A. Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting. J Gen Microbiol. 1988 Aug;134(8):2171–2178. doi: 10.1099/00221287-134-8-2171. [DOI] [PubMed] [Google Scholar]
  19. Pini P. N., Gilbert R. J. A comparison of two procedures for the isolation of Listeria monocytogenes from raw chickens and soft cheese. Int J Food Microbiol. 1988 Dec 31;7(4):331–337. doi: 10.1016/0168-1605(88)90059-1. [DOI] [PubMed] [Google Scholar]
  20. Pusch D. J. A review of current methods used in the United States for isolating Listeria from food. Int J Food Microbiol. 1989 Jun;8(3):197–204. doi: 10.1016/0168-1605(89)90014-7. [DOI] [PubMed] [Google Scholar]
  21. Ralovich B. Data on the enrichment and selective cultivation of listeriae. Int J Food Microbiol. 1989 Jun;8(3):205–217. doi: 10.1016/0168-1605(89)90015-9. [DOI] [PubMed] [Google Scholar]
  22. Skovgaard N., Morgen C. A. Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int J Food Microbiol. 1988 May;6(3):229–242. doi: 10.1016/0168-1605(88)90015-3. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES