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. 1990 Nov;56(11):3537–3545. doi: 10.1128/aem.56.11.3537-3545.1990

Identification of Different Agrobacterium Strains Isolated from the Same Forest Nursery

Marie-France Michel 1, Ana Cristina Miranda Brasileiro 1, Christiane Depierreux 1, Leon Otten 1, Francis Delmotte 1, Lise Jouanin 1,*
PMCID: PMC185018  PMID: 16348358

Abstract

Several Agrobacterium strains isolated from the same forest nursery from 1982 to 1988 were compared by serological, biochemical, and DNA-DNA hybridization methods. Similarities among strains belonging to biovar 2 were observed by indirect immunofluorescence, whereas biovar 1 strains showed serological heterogeneity. Electrophoretic analysis of bacterial envelope-associated proteins showed that few bands appeared in the strains belonging to biovar 1, whereas many proteins appeared in the case of biovar 2 strains. Chromosomal DNA was analyzed with six random C58 chromosomal fragments. None of the six probes hybridized to the DNA of the two biovar 2 strains. One of the probes gave the same hybridization pattern with all biovar 1 strains, whereas the other probes yielded different patterns. The vir regions were closely related in the different pathogenic strains. The T-DNA and replication regions were less conserved and showed some variations among the strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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