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. 1990 Dec;56(12):3748–3754. doi: 10.1128/aem.56.12.3748-3754.1990

Distribution of Hydrogenase Genes in Desulfovibrio spp. and Their Use in Identification of Species from the Oil Field Environment

Gerrit Voordouw 1,*, Vincent Niviere 1, F Grant Ferris 1, Phillip M Fedorak 1, Donald W S Westlake 1
PMCID: PMC185062  PMID: 16348376

Abstract

The distribution of genes for [Fe], [NiFe], and [NiFeSe] hydrogenases was determined for 22 Desulfovibrio species. The genes for [NiFe] hydrogenase were present in all species, whereas those for the [Fe] and [NiFeSe] hydrogenases had a more limited distribution. Sulfate-reducing bacteria from 16S rRNA groups other than the genus Desulfovibrio (R. Devereux, M. Delaney, F. Widdel, and D. A. Stahl, J. Bacteriol. 171:6689-6695, 1989) did not react with the [NiFe] hydrogenase gene probe, which could be used to identify different Desulfovibrio species in oil field samples following growth on lactate-sulfate medium.

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Selected References

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