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. 1990 Dec;56(12):3895–3896. doi: 10.1128/aem.56.12.3895-3896.1990

Sheen Screen, a Miniaturized Most-Probable-Number Method for Enumeration of Oil-Degrading Microorganisms

Edward J Brown 1,*, Joan F Braddock 1
PMCID: PMC185089  PMID: 16348386

Abstract

Sheen Screen is a miniaturized method for enumerating oil-degrading microorganisms. The technique relies on the ability of oil-degrading microorganisms to emulsify oil when provided as a sole carbon source in 24-well tissue culture plates. Sediments that actively respire hydrocarbons have high numbers of Sheen Screen-positive microorganisms.

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Selected References

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  1. Heitkamp M. A., Cerniglia C. E. Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. Appl Environ Microbiol. 1988 Jun;54(6):1612–1614. doi: 10.1128/aem.54.6.1612-1614.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Roubal G., Atlas R. M. Distribution of hydrocarbon-utilizing microorganisms and hydrocarbon biodegradation potentials in Alaskan continental shelf areas. Appl Environ Microbiol. 1978 May;35(5):897–905. doi: 10.1128/aem.35.5.897-905.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Song H. G., Bartha R. Effects of jet fuel spills on the microbial community of soil. Appl Environ Microbiol. 1990 Mar;56(3):646–651. doi: 10.1128/aem.56.3.646-651.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Walker J. D., Colwell R. R. Enumeration of petroleum-degrading microorganisms. Appl Environ Microbiol. 1976 Feb;31(2):198–207. doi: 10.1128/aem.31.2.198-207.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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