Figure 3. Gag expression following injection of HSV-1 amplicon constructs into mice.
HSV-1 amplicon constructs encoding HIV-1 Gag from seven different promoters (Figure 1) were inoculated into the tail base of BALB/c mice (4 mice/group), at a dose of 5 x 105 transducing units. The injection site was marked, and 24 h later, the injection site was recovered with a tissue punch. A tissue lysate was prepared, and subjected to Bradford assay in order to determine the protein concentration. A fixed amount of lysate was then used in a HIV-1 p24 ELISA; the results are expressed as ng of HIV-1 p24 protein per ml of reaction volume, where 1 ml of the reaction contained 2.5 μg of tissue extract. The data shown represent mean values of triplicate measurements; bars denote the standard deviation of those values. The results shown are representative of two independent experiments that yielded very similar data.